Recombinant Human Coagulation Factor X, His-tagged   [ Back  ]                         Price Inquiry

Cat. No.

F10-654H

Product Overview

Recombinant Human coagulation factor X encoding the full length of human coagulation factor X (NP_000495.1) (Met 1- Lys 488) was expressed with a His tag at the C-terminus. It was expressed in Baculovirus.

Description

Coagulation factor X, also known as FX, F10, Eponym Stuart-Prower factor, and thrombokinase, is an enzyme of the coagulation cascade. Factor X is synthesized in the liver as a mature heterodimer formed from a single-chain precursor, and vitamin K is essential for its synthesis. Factor X is activated into factor Xa by both factor IX (with its cofactor, factor VIII in a complex known as intrinsic Xase) and factor VII (with its cofactor, tissue factor in a complex known as extrinsic Xase) through cleaving the activation propeptide. As the first member of the final common pathway or thrombin pathway, acitivated factor X converts prothrombin to thrombin in the presence of factor Va, Ca2+, and phospholipid during blood clotting and cleaves prothrombin in two places (an arg-thr and then an arg-ile bond).  

Source

Baculovirus.

Purity

>97 % as determined by SDS-PAGE.

Endotoxin

< 1.0 EU per 1μg cytokine as determined by the LAL method.

Stability

Samples are stable for up to twelve months from date of receipt -70⁰C.

Predicted N Terminal

Asn32.

Molecular Mass

The recombinant human coagulation factor X consists of 468 amino acids and predicts a molecular mass of 52.8 kDa. As a result of glycosylation, it migrates with the apparent molecular mass of approximately 48 and 22 kDa corresponding to the cleaved light and heavy chain respectively in SDS-PAGE under reducing conditions.

Formulation

Supplied as a 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH8, 10% glycerol.

Reconstitution

Follow the instructions on the vial. Centrifuge the vial at 4⁰Cbefore opening to recover the entire contents.

Storage

Store it under sterile conditions at -70⁰C. It is recommended that the protein be aliquoted for optimal storage and be used as soon as possible. Avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

F10 coagulation factor X [ Homo sapiens ]

Synonyms

F10; coagulation factor X; FX; FXA; Coagulation factor X; Stuart factor; Stuart-Prower factor; Factor X light chain; Factor X heavy chain; Activated factor Xa heavy chain; EC 3.4.21.6; OTTHUMP00000018735; prothrombinase

GeneID

2159

mRNA Refseq

NM_000504

Protein Refseq

NP_000495

MIM

227600

UniProt ID

P00742

Chromosome Location

13q34

Pathway

Complement and coagulation cascades; Hemostasis; Metabolism of proteins

Function

calcium ion binding; peptidase activity; phospholipid binding; protein binding; serine-type endopeptidase activity