|
Cat. No. |
CAB549 |
|
Antigen Information |
Tubulin folding
cofactor A, also known as TBCA, is one of four proteins (cofactors
A, D, E, and C) involved in the pathway leading to correctly folded
beta-tubulin from folding intermediates. Cofactors A and D are
believed to play a role in capturing and stabilizing beta-tubulin in
a quasi-native confirmation. This protein is essential for cell
viability and its knockdown produces a decrease in the amount of
soluble tubulin, modifications in microtubules and G1 cell cycle
arrest. |
|
Immunogen |
Recombinant TBCA
(1-108aa) purified from E. coli. |
|
Isotype |
Mouse IgG2b heavy
chain and k light chain. |
|
Clone |
Anti-human TBCA mAb,
clone AT1A5, is derived from hybridization of mouse F0 myeloma cells
with spleen cells from BALB/c mice immunized with a recombinant
human TBCA protein. |
|
Concentration |
1mg/ml. |
|
Form |
Liquid. In
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
|
Application |
ELISA, WB. |
|
Storage |
Can be stored at
+4°C short term (1-2 weeks). For long term storage, aliquot and
store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
|
Usage |
The antibody has
been tested by ELISA and Western blot analysis to assure specificity
and reactivity. Since application varies, however, each
investigation should be titrated by the reagent to obtain optimal
results. Recommended dilution range for Western blot analysis is
1:1000 ~ 2000. Recommended starting dilution is 1:1000. |
|
Western Blot
Analysis |
Cell
lysates of LNCaP(20ug) were resolved by SDS-PAGE, transferred to NC
membrane and probed with anti-human TBCA (1:1000). Proteins were
visualized using a goat anti-mouse secondary antibody conjugated to
HRP and an ECL detection system. |
|
Gene Information
|
Gene Name |
TBCA tubulin folding cofactor A [ Homo sapiens ] |
|
Synonyms
|
tubulin folding
cofactor A; TBCA; tubulin-specific chaperone a; chaperonin cofactor
A; CFA; TCP1-chaperonin cofactor A; Tubulin-folding cofactor A |
|
GeneID |
6902 |
|
mRNA Refseq |
NM_004607 |
|
Protein Refseq |
NP_004598 |
|
MIM |
610058 |
|
UniProt ID |
O75347 |
|
Chromosome Location |
5q14.1 |
|
Pathways |
Metabolism of
proteins |
|
Function |
chaperone binding;
unfolded protein binding |
|
PDB rendering based on 1h7c.
|
Download Datasheet:
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