|
Cat. No. |
CAB546 |
|
Antigen Information |
Lipopolysaccharide-induced
tumor necrosis factor-alpha factor(LITAF) mediates the expression of
inflammatory cytokines such as TNF-alpha in Lipopolysaccharide-induced
processes. LITAF binds to STAT6B, a member of the STAT6 family
forming a complex on the TNF-alpha promoter that modulates TNF
activity. High levels of expression of LITAF mRNA have been observed
predominantly in the placenta, peripheral blood leukocytes, lymph
nodes and spleen. |
|
Immunogen |
Recombinant human
LITAF (1-161aa) purified from E. coli. |
|
Isotype |
Mouse IgG1 heavy
chain and k light chain. |
|
Clone |
Anti-human LITAF
mAb, clone AT1F9, is derived from hybridization of mouse F0 myeloma
cells with spleen cells from BALB/c mice immunized with a
recombinant human LITAF protein. |
|
Concentration |
1mg/ml. |
|
Form |
Liquid. In
Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
|
Application |
ELISA, WB. |
|
Storage |
Can be stored at
+4°C short term (1-2 weeks). For long term storage, aliquot and
store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
|
Usage |
The antibody has
been tested by ELISA and Western blot analysis to assure specificity
and reactivity. Since application varies, however, each
investigation should be titrated by the reagent to obtain optimal
results. Recommended dilution range for Western blot analysis is
1:2000 ~ 3000. Recommended starting dilution is 1:2000. |
|
Western Blot
Analysis |
Cell lysates of HeLa(20ug) were
resolved by SDS-PAGE, transferred to NC membrane and probed with
anti-human LITAF (1:2000). Proteins were visualized using a goat
anti-mouse secondary antibody conjugated to HRP and an ECL detection
system.
|
|
Gene Information
|
Gene Name |
LITAF lipopolysaccharide-induced TNF factor [ Homo sapiens ] |
|
Synonyms
|
lipopolysaccharide-induced TNF factor; PIG7; FLJ38636; MGC116698;
LITAF; MGC125276; MGC116700; CMT1C; MGC116701; MGC125274; SIMPLE;
TP53I7; MGC125275; lipopolysaccharide-induced TNF-alpha factor;
p53-induced gene 7 protein; LPS-induced TNF-alpha factor; tumor
protein p53 inducible protein 7; small integral membrane protein of
lysosome/late endosome;
lipopolysaccharide-induced tumor necrosis factor-alpha factor ;
Small integral membrane protein of lysosome/late endosome |
|
GeneID |
9516 |
|
mRNA Refseq |
NM_001136472 |
|
Protein Refseq |
NP_001129944 |
|
MIM |
603795 |
|
UniProt ID |
Q99732 |
|
Chromosome Location |
16p13.13 |
|
Function |
RNA polymerase II
transcription factor activity; WW domain binding; signal transducer
activity |
|
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