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Cat. No. |
Wnt3a-1569M |
|
Product Overview |
Recombinant murine Wnt-3a is a monomeric glycoprotein
containing 328 amino acid residues expressed in cell
culture. MW=38.0-41.0kDa. |
|
Description |
Wnt-3a belongs to the Wnt family of signaling proteins that
play a key role in maintaining the integrity of embryonic
and adult tissues. Expression of Wnt-3a occurs primarily
along the dorsal midline across overlapping regions of the
Central Nervous System (CNS). Wnt-3a signaling is essential
for various morphogenetic events including embryonic
patterning, cell determination, cell proliferation, CNS
development, and cytoskeletal formation. Like other members
of this family, Wnt-3a contains a highly conserved lipid
modified cysteine rich domain that is essential for cell
signaling. During a biochemical process called the canonical
Wnt pathway; Wnt family members bind to and activate
seven-pass transmembrane receptors of the Frizzled family
ultimately leading to the disruption of beta-Catenein
degradation. Intracellular accumulation of beta-Catenin
increases translocation of the protein into the nucleus
where it binds to TCF/LEF transcription factors to promote
gene expression. Lack of Wnt signaling disrupts
transcriptional activation of tumor suppressor genes and has
shown to result in neoplastic transformation, oncogenesis,
and human degenerative diseases. |
|
Source |
cell culture. |
|
Sequence |
VGPQYSS LSTQPILCAS IPGLVPKQLR FCRNYVEIMP SVAEGVKAGI
QECQHQFRGR RWNCTTVSNS LAIFGPVLDK ATRESAFVHA IASAGVAFAV
TRSCAEGSAA ICGCSSRLQG SPGEGWKWGG CSEDIEFGGM VSREFADARE
NRPDARSAMN RHNNEAGRQA IASHMHLKCK CHGLSGSCEV KTCWWSQPDF
RTIGDFLKDK YDSASEMVVE KHRESRGWVE TLRPRYTYFK VPTERDLVYY
EASPNFCEPN PETGSFGTRD RTCNVSSHGI DGCDLLCCGR GHNARTERRR
EKCHCVFHWC CYVSCQECTR VYDVHTCK. |
|
Reactivity |
N/A. |
|
Purity |
Greater than 75% by SDS-PAGE gel and HPLC analyses.
Endotoxin level is less than 0.1 ng per μg (1EU/μg). |
|
Specific Activity |
N/A. |
|
Preparation |
The ED50 was determined by its ability to induced alkaline
phosphatase production by CCL - 226 cells. The expected ED50
for this effect is 0.4 - 0.6 ng/ml. |
|
Tested Application |
N/A. |
|
Storage |
The lyophilized WNT-3a recombinant protein is stable for at
least 2 years from date of receipt at -20˚C. Reconstituted
WNT-3a is stable for at least 3 months when stored in
working aliquots with a carrier protein at -20˚C. As with
any protein, exposing WNT-3a recombinant protein to repeated
freeze / thaw cycles is not recommended. When working with
proteins care should be taken to keep recombinant protein at
a cool and stable temperature. |
|
Gene Information
|
Gene Name |
Wnt3a wingless-related MMTV integration site 3A [ Mus
musculus ] |
|
Synonyms |
Wnt3a; wingless-related MMTV integration site 3A; vt;
Wnt-3a; vestigial tail; OTTMUSP00000005961 |
|
GeneID |
22416 |
|
mRNA Refseq |
NM_009522 |
|
Protein Refseq |
NP_033548 |
|
UniProt ID |
P27467 |
|
Chromosome Location |
11 32.0 cM |
|
Pathway |
Basal cell carcinoma; Hedgehog signaling pathway;
Melanogenesis; Pathways in cancer; Wnt signaling pathway |
|
Function |
receptor binding; protein binding; signal transducer
activity |
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Abbr.: 
