|
Cat. No. |
B4GALT1-706H |
|
Description |
Beta-1,4-galactosyltransferase 1, also known as B4GALT1, GGTB2 and
beta4GalT1, is a single-pass type II membrane protein. B4GALT1 is a
member of the glycosyltransferase 7 family. It is ubiquitously
expressed but at very low levels in fetal and adult brain. By
sequence similarity, the beta4GalTs form four groups: beta4GalT1 and
beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6,
and beta4GalT7. B4GALT1 is an enzyme that functions in the synthesis
of glycoconjugates It has two flexible loops, one short and one
long. Upon binding a metal ion and UDP-galactose, the loops change
from an open to a closed conformation, repositioning residues to
lock the ligands in place. B4GALT1 is one of the best-studied
glycosyltransferases. It plays a key role in the synthesis of
selectin ligands. B4GALT1 may play an important role in the
inflammation reaction. Defects in B4GALT1 are the cause of
congenital disorder of glycosylation type 2D (CDG2D). CDGs are a
family of severe inherited diseases caused by a defect in protein N-glycosylation. |
|
Source |
Saccharomyces
cerevisiae. |
|
Form |
powder. |
|
Color |
slightly yellow. |
|
Foreign Activity |
β-galactosidase
≤0.1%. |
|
Components |
contains Tris
buffer salts and BSA. |
|
Other Notes |
Enzyme catalyst for
the stereoselective synthesis of galactosyl carbohydrates1,2,3,4. |
|
Unit Definition |
1 U correspond to
the amount of enzyme which transfers 1 μmol galactose from
UDP-galactose to D-glucose per minute at pH 8.4 and 30°C in the
presence of α-lactalbumin. |
|
Storage Temp |
−20°C. |
|
Gene Information
|
Gene Name |
B4GALT1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase,
polypeptide 1 [ Homo sapiens ] |
|
Synonyms
|
B4GALT1;
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1;
GT1; GTB; CDG2D; GGTB2; B4GAL-T1; MGC50983; beta4Gal-T1;
DKFZp686N19253; B4GALT1; beta-1,4-galactosyltransferase 1;
beta-1,4-GalTase 1; glycoprotein-4-beta-galactosyltransferase 2;
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1;
UDP-galactose:beta-N-acetylglucosamine
beta-1,4-galactosyltransferase 1; EC 2.4.1.22; EC 2.4.1.38; EC
2.4.1.90 |
|
GeneID |
2683 |
|
mRNA Refseq |
NM_001497 |
|
Protein Refseq |
NP_001488 |
|
MIM |
137060 |
|
UniProt ID |
P15291 |
|
Chromosome Location |
9p13 |
|
Pathways |
Galactose
metabolism; Glycosaminoglycan biosynthesis - keratan sulfate;
Glycosphingolipid biosynthesis - lacto and neolacto series;
Metabolic pathways; N-Glycan biosynthesis |
|
Function |
N-acetyllactosamine
synthase activity; UDP-galactosyltransferase activity;
beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
activity; cytoskeletal protein binding; lactose synthase activity;
metal ion binding; transferase activity, transferring glycosyl
groups |
|
PDB rendering based on 1nf5.
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Abbr.: 
