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Cat. No. |
P34 Protein-193S |
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Product Overview |
Recombinant Soybean P34 Protein epitopes expressed in E.Coli
contains Soybean (Glycine max) P34 Protein epitopes 214-261,
351-379 amino acids. The protein is fused to a GST tag at
N-terminus. |
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Description |
The P34 protein is the main allergen for soybean sensitive
humans. Soybean protein P34, a thiol protease belonging to
the papain family, is a monomeric allergen having an
N-terminal amino acid sequence and amino acid composition
identical to that of the seed 34kDa protein. It is an
insoluble glycoprotein having a pI of 4.5 and a calculated
mass of 28.643 Dalton, representing 2–3% of total soybean
protein. Upon glycosylation, the mass will be somewhat
larger, resulting in a 32kDa band in non-reduced SDS PAGE
gels. It exhibits no enzymatic function due to an absence of
the catalytic cysteine. P34 is stored in storage vacuoles of
soybean cotyledons. |
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Source |
E.Coli. |
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Physical Appearance |
Use as an antigen in ELISA and Western Blots. |
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Formulation |
50mM Tris-HCl, pH 8.0, 60mM NaCl, 10mM glutathione and 50%
glycerol. |
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Stability |
Five years frozen. One month in solution at room
temperature. |
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Purity |
Protein is >95% pure as determined by 10% PAGE (coomassie
staining) and RP-HPLC. |
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Storage |
Protein is shipped at ambient
temperature. Upon arrival, Store at -20oC |
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Gene Information
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Gene Name |
LOC548062 34 kDa maturing seed vacuolar thiol protease
precursor [ Glycine max ] |
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Synonyms |
P34 Protein; 34 kDa maturing
seed vacuolar thiol protease precursor; P34 probable thiol
protease;
EC=3.4.22.- |
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GeneID |
548062 |
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UniProt ID |
P22895 |
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Abbr.: 
