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Description |
The CCAAT-box
Binding Protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich
transcription activator. It selectively activates transcription
of genes that contain GCCAAT consensus sequence in promoter
region, including human Ha-Ras, alpha-globin, hsp70 and many
other cellular and viral genes. Cloning and functional
characterization of the protein revealed that CTF1 contains a
N-terminal DNA binding domain and a C-terminal proline-rich
activation domain. CTF1 was shown to interact with the general
transcription factor IIB (TFIIB) and facilitate the recruitment
of TFIIB to TBP-DNA complexes, with the forkhead thyroid
transcription factor TTF2 to regulate hormonedependent
transcription of thyroperoxidase gene, as well as other receptor
proteins to synergize hormone receptor-responsive activation.
The proline-rich activation domain of human CTF1 protein (amino
acids 399-499) is isolated from an E. coli strain that carries
the coding sequence of the fused protein under the control of a
T7 promoter. GST-CTF1 has been used for protein-protein
interaction assays. |
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