Recombinant Rat Chemokine (C-C Motif) Ligand 4   [ Back  ]                         Price Inquiry

Cat. No.

Ccl4-106R

Product Overview

Recombinant rat MIP-1 beta produced in E.coli is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

Description

Both MIP-1 alpha and MIP-1 beta are structurally and functionally related CC chemokines. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. While both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes their effect on lymphocytes differ; with MIP-1 alpha selectively attracting CD8+ lymphocytes and MIP-1 beta selectively attracting CD4+ lymphocytes. Additionally, MIP-1 alpha and MIP-1 beta have also been shown to be potent chemoattractants for B cells, eosinophils and dendritic cells. Both human and murine MIP-1 alpha and MIP-1 beta are active on human and murine hematopoietic cells.

Source

E.coli.

Purity

> 98% by SDS-PAGE and HPLC analysis.

Endotoxin level

< 0.1 ng/μg of MIP-1 beta.

Stabilizer

None.

Formulation

Lyophilized.

Biologically Activity

Determined by its ability to chemoattract human monocytes using a concentration range of 0.01-1.0 μg/ml.

Reconstitution

The rat MIP-1 beta should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted in water or other buffer solutions or stored at -20°C.

Stability

The lyophilized protein is stable for a few weeks at room temperature, but best stored at -20°C. Reconstituted rat MIP-1 beta should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

Ccl4 chemokine (C-C motif) ligand 4 [ Rattus norvegicus ]

Synonyms

Ccl4; chemokine (C-C motif) ligand 4; Scya4; Mip1-b; small inducible cytokine A4; macrophage inflammatory protein-1 beta

GeneID

116637

mRNA Refseq

NM_053858

Protein Refseq

NP_446310

UniProt ID

P50230

Chromosome Location

q26

Pathway

Chemokine signaling pathway; Cytokine-cytokine receptor interaction

Function

chemokine activity; protein binding