|
Cat. No. |
TIMP2-880H |
|
Product Overview |
Recombinant Human TIMP2
encoding the mature form of human TIMP2 (NP_003246.1) ( Cys
27- Pro 220) was expressed in
HEK293 with the fused Fc region of human IgG1 at the
N-terminus. |
|
Description |
Tissue inhibitors of metalloproteinases (TIMP) family are
natural inhibitors of the matrix metalloproteinases (MMPs),
the zinc enzymes involved in extracellular matrix
maintenance and remodeling. The TIMP family encompasses four
members (TIMP1-4), and they inhibit most MMPs by forming
non-covalent binary complex. TIMP2 is a 22 kDa non N-glycosylated
protein expressed by a variety of cell types, and plays a
unique role among TIMP family members owing to its functions
to regulate cellular responses to growth factors. Findings
establish an unexpected, MMP-independent mechanism for TIMP2
inhibition of endothelial cell proliferation in vitro and
reveal an important component of the antiangiogenic effect
of TIMP2 in vivo. TIMP-2 thus is critical to the maintenance
of tissue homeostasis and is involved in the regulation of
tumor microenvironment. |
|
Molecular Mass |
The recombinant human Fc/TIMP2 is a disulfide-linked
homodimeric protein. The reduced monomer consists of 431
amino acids and has a predicted molecular mass of 48 kDa as
estimated in SDS-PAGE under reducing conditions. |
|
Source |
Human Cells (HEK293). |
|
Predicted N Terminal |
Glu 20. |
|
Endotoxin |
< 1.0 EU per 1μg cytokine as determined by the LAL method. |
|
Formulation |
Supplied as a 0.2μm filtered solution of 100mM Glycine, 10mM
NaCl, 50mM Tris, pH7.5. |
|
Reconstitution |
Follow the instructions on the vial. Centrifuge the vial at
4℃ before opening to recover the entire contents. |
|
Purity |
> 95%, as determined by SDS-PAGE. |
|
Stability |
Samples are stable for up to twelve months from date of
receipt -70℃. |
|
Storage |
Store it under sterile conditions at -70℃. It is recommended
that the protein be aliquoted and be used as soon as
possible. Avoid repeated freeze-thaw cycles. |
|
Gene Information
|
Gene Name |
TIMP2 TIMP metallopeptidase inhibitor 2 [ Homo sapiens ] |
|
Synonyms |
TIMP2; TIMP metallopeptidase inhibitor 2; CSC-21K; tissue
inhibitor of metalloproteinase 2; tissue inhibitor of
metalloproteinases 2; TIMP-2 |
|
GeneID |
7077 |
|
mRNA Refseq |
NM_003255 |
|
Protein Refseq |
NP_003246 |
|
MIM |
188825 |
|
UniProt ID |
P16035 |
|
Chromosome Location |
17q25 |
|
Function |
enzyme activator activity; enzyme inhibitor activity;
integrin binding; metalloendopeptidase inhibitor activity;
protein binding |
|
PDB rendering based on 1bqq.
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