|
Cat. No. |
HMGB1-752H |
|
Product
Overview |
Recombinant Human HMGB1
(NP_002119.1) (Met 1- Glu 215) was expressed in
HEK293 with
the fused Fc region of human IgG1 at the N-terminus. |
|
Description |
Human
High-mobility group box 1 protein (HMGB1), also known as
HMG-1 or amphoterin previously, is a member of the HMGB
family consisting of three members, HMGB1, HMGB2 and HMGB3.
HMGB1 is a non- histone architectural chromosomal protein
ubiquitously present in all vertebrate nuclei and binds
double-stranded DNA without sequence specificity. HMGB1 is
structurally composed of two homologous DNA-binding domains
entitled box A and box B and a highly negative-charged C
terminus. This protein is extremely conserved across species
with 99- 100% aa sequence homology to mouse, rat, bovine,
canine, porcine proteins. As a nuclear protein, HMGB1
stabilizes nucleosomes and allows bending of DNA that
facilitates gene transcription which is essential for
individual survival. Meanwhile, it is revealed that HMGB1
can also act as a cytokine extracellularlly and regulates
monocyte, T cell, dendritic cell activities in inflammatory
responses. Once secreted, HMGB1 induces signal transduction
through its receptor RAGE and perhaps TLR2 and TLR4. The
immune-regulatory function of HMGB1 may lead to being
identified as new therapeutic target. |
|
Molecular
Mass |
The
recombinant human HMGB1/Fc is a disulfide-linked homodimeric
protein.The reduced monomer consists of 452 amino acids and
has a predictedmolecular mass of 51.5 kDa. As a result of
glycosylation, the apparent molecular mass of rhHMGB1/Fc
monomer is approximately 55-60 kDa in SDS-PAGE under
reducing conditions. |
|
Source |
Human Cells
(HEK293). |
|
Predicted N
Terminal |
Glu 20. |
|
Endotoxin |
<1.0 EU per
1μg cytokine as determined by the LAL method. |
|
Formulation |
Supplied as
a 0.2μm filtered solution of 100mM Glycine, 10mM NaCl, 50mM
Tris, pH7.5. |
|
Reconstitution |
Follow the
instructions on the vial. Centrifuge the vial at 4℃ before
opening to recover the entire contents. |
|
Purity |
> 97% as
determined by SDS-PAGE. |
|
Stability |
Samples are
stable for up to twelve months from date of receipt -70℃. |
|
Storage |
Store it
under sterile conditions at -70℃immediately after receipt.
It is recommended that the protein be aliquoted and be used
as soon as possible. Avoid repeated freeze-thaw cycles. |
|
Gene
Information
|
Gene Name |
HMGB1 high-mobility group box 1 [ Homo sapiens ] |
|
Synonyms
|
HMGB1;
high-mobility group box 1; HMG1; HMG3; SBP-1;
DKFZp686A04236; Amphoterin; OTTHUMP00000018196;
OTTHUMP00000190860; OTTHUMP00000200117; high mobility group
box 1; high mobility group protein 1; Sulfoglucuronyl
carbohydrate binding protein; high-mobility group
(nonhistone chromosomal) protein 1; High mobility group
protein B1; HMG-1 |
|
GeneID |
3146 |
|
mRNA Refseq |
NM_002128 |
|
Protein
Refseq |
NP_002119 |
|
MIM |
163905 |
|
UniProt ID |
P09429 |
|
Chromosome
Location |
13q12 |
|
Pathway |
Base
excision repair |
|
Function |
DNA bending
activity; transcription factor binding |
|
PDB rendering based on 1aab.
|
Download Datasheet:
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Abbr.: 
