|
Cat. No. |
NBL1-682H |
|
Product
Overview |
Recombinant
Human
neuroblastoma, suppression of tumorigenicity 1 encoding the
human DAN precursor (NP_005371.1) (Met1-Asp180) was produced
in HEK293. It was expressed with a C-terminal his tag. |
|
Description |
Human
differential screening-selected gene aberrative in
neuroblastoma (DAN) originally isolated from a normal lung
cDNA library, is a prototypical member of the DAN family of
secreted glycoproteins. There are at least five mammalian
protein members in the evolutionarily conserved Dan family
including DAN, Gremlin/DRM, Cer1 (Cerberus-related), Dante
and PRDC (protein related to DAN and cereberus), and share
the C-terminal cystine-knot motif. As a secreted
glycoprotein, DAN acts as the BMPs (bone morphogenetic
protein) antagonist by binding to BMPs and preventing their
interactions with signaling receptor complexes, and
accordingly regulates the processes of embryonic development
and tissue differentiation. In addition, it has been
reported that DAN also acts as an inhibitor of cell cycle
progression and monocyte chemotaxis, and is closely involved
in human neuroblastoma differentiation. DAN thus is regarded
as a novel candidate of tumour suppressor. |
|
Molecular
Mass |
The
recombinant human DAN consists of 176 amino acids and has a
calculated molecular mass of 19.3KDa. As a result of
glycosylation, rh DAN migrates as an approximately 27KDa
protein in SDS-PAGE under reducing conditions. |
|
Source |
Human Cells
(HEK293). |
|
Predicted N
Terminal |
Ala 16. |
|
Endotoxin |
<1.0EU per
1g cytokine as determined by the LAL method. |
|
Formulation |
Supplied as
a 0.2m filtered solution of PBS, pH7.4. |
|
Reconstitution |
Follow the
instructions on the vial. Centrifuge the vial at 4℃ before
opening to recover the entire contents. |
|
Purity |
>98%, as
determined by SDS-PAGE. |
|
Stability |
Samples are
stable for up to twelve months from date of receipt -70℃. |
|
Storage |
Store it
under sterile conditions at -70℃.
It is recommended that the protein be aliquoted and be used
as soon as possible. Avoid repeated freeze-thaw cycles. |
|
Gene
Information
|
Gene Name |
NBL1 neuroblastoma, suppression of tumorigenicity 1 [ Homo
sapiens ] |
|
Synonyms
|
NBL1;
neuroblastoma, suppression of tumorigenicity 1; NB; DAN;
NO3; DAND1; MGC8972; D1S1733E; neuroblastoma suppressor of
tumorigenicity 1; differential screening-selected gene
aberrant in neuroblastoma; neuroblastoma candidate region,
suppression of tumorigenicity 1; Neuroblastoma suppressor of
tumorigenicity 1; Zinc finger protein DAN; DAN domain family
member 1 |
|
GeneID |
4681 |
|
mRNA Refseq |
NM_005380 |
|
Protein
Refseq |
NP_005371 |
|
MIM |
600613 |
|
UniProt ID |
P41271 |
|
Chromosome
Location |
1p36.3-p36.2 |
|
|
Download Datasheet:
|
|
Abbr.: 
