|
Cat. No. |
IFNA4-29H |
|
Product
Overview |
Recombinant
Human IFNA4 encoding the mature form of human IFN-α 4b
(NP_066546.1) (Cys 23- Asp 189) was expressed with the
N-terminal fused Fc region of human IgG1. It was produced in
HEK293. |
|
Description |
Interferons
(IFNs) are natural glycoproteins belonging to the cytokine
superfamily, and are produced by a wide variety of cells
types in response to challenges by foreign agents such as
viruses, parasites and tumor cells. There are three major
classes of IFNs according to the receptor type through which
they signal, and the type I IFNs bind to a specific receptor
complex known as the IFN-α receptor (IFNAR) consisting of
IFNAR1 and IFNAR2 chains. The type I IFNs present in humans
are IFN-α, IFN-β and IFN-ω, and the IFN-α come in 13
subtypes whose encoding genes locate in a cluster on
chromosome 9. IFN-α is produced by leukocytes and mainly
involved in innate immune response against viral infection
together with other IFNs. IFN-α is produced in response to
the presence of dsRNA, a key indicator of viral infection
via Toll Like Receptors, and assists the immune response by
inhibiting viral replication within host cells, activating
natural killer cells and macrophages, increasing antigen
presentation to lymphocytes, and inducing the resistance of
host cells to viral infection. |
|
Molecular
Mass |
The
recombinant human Fc/ IFN-α 4b is a disulfide-linked
homodimeric protein. The reduced monomer consists of 403
amino acids and has a predicted molecular mass of 46 kDa. As
a result of glycosylation, the apparent molecular mass of rh
Fc/IFN-α 4b monomer is approximately 50 kDa in SDS-PAGE
under reducing conditions. |
|
Source |
Human Cells
(HEK293). |
|
Predicted N
Terminal |
Ser 22. |
|
Endotoxin |
<1.0 EU per
1μg cytokine as determined by the LAL method. |
|
Formulation |
Supplied as
a 0.2μm filtered solution of 100mM Glycine, 10mM NaCl, 50mM
Tris, pH7. |
|
Reconstitution |
Follow the
instructions on the vial. Centrifuge the vial at 4℃
before opening to recover the entire contents. |
|
Purity |
> 97% as
determined by SDS-PAGE. |
|
Stability |
Samples are
stable for up to twelve months from date of receipt -70℃. |
|
Storage |
Store it
under sterile conditions at -70℃.
It is recommended that the protein be aliquoted for optimal
storage and usage. Avoid repeated freeze-thaw cycles |
|
Gene
Information
|
Gene Name |
IFNA4 interferon, alpha 4 [ Homo sapiens ] |
|
Synonyms
|
IFNA4;
interferon, alpha 4; INFA4; MGC142200; Interferon alpha-4;
Interferon alpha-4B; Interferon alpha-M1; Interferon
alpha-76; OTTHUMP00000021135; OTTHUMP00000045102 |
|
GeneID |
3441 |
|
mRNA Refseq |
NM_021068 |
|
Protein
Refseq |
NP_066546 |
|
MIM |
147564 |
|
UniProt ID |
P05014 |
|
Chromosome
Location |
9p22 |
|
Pathway |
Jak-STAT
signaling pathway; Natural killer cell mediated
cytotoxicity; RIG-I-like receptor signaling pathway;
Regulation of autophagy; Toll-like receptor signaling
pathway; Antigen processing and presentation; Autoimmune
thyroid disease; Cytokine-cytokine receptor interaction |
|
Function |
cytokine
activity; interferon-alpha/beta receptor binding |
|
|
Download Datasheet:
|
|
Abbr.: 
