|
Cat. No. |
F10-289H |
|
Product
Overview |
Recombinant
Human coagulation factor X encoding the full length of human
coagulation factor X (NP_000495.1) (Met 1- Lys 488) was
expressed with a his tag at the C-terminus. It was expressed
in Baculovirus. |
|
Description |
Coagulation
factor X, also known as FX, F10, Eponym Stuart-Prower
factor, and thrombokinase, is an enzyme of the coagulation
cascade. Factor X is synthesized in the liver as a mature
heterodimer formed from a single-chain precursor, and
vitamin K is essential for its synthesis. Factor X is
activated into factor Xa by both factor IX (with its
cofactor, factor VIII in a complex known as intrinsic Xase)
and factor VII (with its cofactor, tissue factor in a
complex known as extrinsic Xase) through cleaving the
activation propeptide. As the first member of the final
common pathway or thrombin pathway, acitivated factor X
converts prothrombin to thrombin in the presence of factor
Va, Ca2+, and phospholipid during blood clotting and cleaves
prothrombin in two places (an arg-thr and then an arg-ile
bond). |
|
Molecular
Mass |
The
recombinant human coagulation factor X consists of 468 amino
acids and predicts a molecular mass of 52.8 kDa. As a result
of glycosylation, it migrates with the apparent molecular
mass of approximately 48 and 22 kDa corresponding to the
cleaved light and heavy chain respectively in SDS-PAGE under
reducing conditions. |
|
Source |
Baculovirus. |
|
Predicted N
Terminal |
Asn32. |
|
Purity |
> 97% as
determined by SDS-PAGE. |
|
Formulation |
Supplied as
a 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH8, 10%
glycerol. |
|
Stability |
Samples are stable for up to
twelve months from date of receipt -70℃. |
|
Endotoxin |
< 1.0 EU
per 1μg cytokine as determined by the LAL method. |
|
Reconstitution |
Follow the instructions on the
vial. Centrifuge the vial at 4℃
before opening to recover the entire contents. |
|
Storage |
Store it
under sterile conditions at -70℃. It is recommended that the
protein be aliquoted for optimal storage and be used as soon
as possible. Avoid repeated freeze-thaw cycles. |
|
Gene
Information
|
Gene Name |
F10 coagulation factor X [ Homo sapiens ] |
|
Synonyms
|
F10;
coagulation factor X; FX; FXA; Coagulation factor X;
Stuart factor; Stuart-Prower
factor; Factor X light chain; Factor X heavy chain;
Activated factor Xa heavy chain; EC 3.4.21.6;
OTTHUMP00000018735; prothrombinase |
|
GeneID |
2159 |
|
mRNA Refseq |
NM_000504 |
|
Protein
Refseq |
NP_000495 |
|
MIM |
227600 |
|
UniProt ID |
P00742 |
|
Chromosome
Location |
13q34 |
|
Pathway |
Complement
and coagulation cascades; Hemostasis; Metabolism of proteins |
|
Function |
calcium ion
binding; peptidase activity; phospholipid binding; protein
binding; serine-type endopeptidase activity |
|
PDB rendering based on 1c5m.
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Abbr.: 
