Recombinant Human DDC, His-tagged   [ Back  ]                         Price Inquiry

Cat. No.

DDC-284H

Product Overview

Recombinant Human DDC encoding  the full length of human DDC (NP_000781.1) (Met 1- Glu 480) was expressed with a his tag at the C-terminus. It was expressed in Baculovirus.

Description

Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.   

Molecular Mass

The recombinant human DDC consists of 490 amino acids and predicts a molecular mass of 55 kDa. It migrates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.  

Source

Baculovirus.

Predicted N Terminal

Met.

Purity

> 90%, as determined by SDS-PAGE.

Formulation

Supplied as a 0.2μm filtered solution of 50mM Tris, 100mM NaCl, pH8, 10% glycerol.

Stability

Samples are stable for up to twelve months from date of receipt -70℃.

Endotoxin

< 1.0 EU per 1μg cytokine as determined by the LAL method.

Reconstitution

Follow the instructions on the vial. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Storage

Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage and be used as soon as possible. Avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

DDC dopa decarboxylase (aromatic L-amino acid decarboxylase) [ Homo sapiens ]

Synonyms

DDC; dopa decarboxylase (aromatic L-amino acid decarboxylase); AADC; Aromatic-L-amino-acid decarboxylase; DOPA decarboxylase; EC 4.1.1.28; L-Dopa decarboxylase; aromatic L-amino acid decarboxylase

GeneID

1644

mRNA Refseq

NM_000790

Protein Refseq

NP_000781

MIM

107930

UniProt ID

P20711

Chromosome Location

7p11

Pathway

Betalain biosynthesis; Biosynthesis of alkaloids derived from shikimate pathway; Histidine metabolism; Isoquinoline alkaloid biosynthesis; Metabolic pathways; Metabolic pathways; Phenylalanine metabolism; Tryptophan metabolism; Synaptic Transmission

Function

aromatic-L-amino-acid decarboxylase activity; lyase activity; protein binding; pyridoxal phosphate binding