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Cat. No. |
CA10-258H |
|
Product
Overview |
Recombinant Human carbonic
anhydrase X was produced in
Human Cells
(HEK293). The mature form of human CARPX (NP_001076002.1)
(Met1-Asn300) was obtained after cleavage of the C-terminal
His-tagged human IgG1 Fc region from the C-terminus of the
purified chimeric protein. |
|
Description |
Carbonic
anhydrase X, also called carbonic anhydrase - related
protein X (CARPX) and CA10, belongs to the CA family of zinc
metalloenzymes which catalyze the reversible hydration of
carbon dioxide in various biological processes such as
respiration, renal tubular acidification and bone resorption.
The secreted protein CARPX without CA activity (hydration of
CO2) is identified as an acatalytic member of the
alpha-carbonic anhydrase subgroup. CARP X expression is
detected in the adult total brain and almost all parts of
the central nervous system, but not in the fetal brain.
Accordingly, CARP X is suggested to play a role in the
development of central nervous system, especially the brain.
The same CARP X protein are encoded by multiple transcript
variants of this gene. |
|
Molecular
Mass |
The
recombinant human CARPX consists of 285 amino acids and has
a predicted molecular mass of 27.2kDa. Due to glycosylation,
the rhCARPX migrates as an approximately 38kDa band in SDS-PAGE
under reducing conditions. |
|
Source |
Human Cells
(HEK293). |
|
Predicted N
Terminal |
Gln 22. |
|
Endotoxin |
<1.0EU per
1μg cytokine as determined by the LAL method. |
|
Formulation |
Supplied as
a 0.2μm filtered solution of 100mM Glycine, 10mM NaCl, 50mM
Tris, pH7.5. |
|
Reconstitution |
Sample is ready to use.
Centrifuge the vial at 4℃
before opening to recover the entire contents. |
|
Purity |
>95%, as
determined by SDS-PAGE. |
|
Stability |
Samples are
stable for up to twelve months from date of receipt -70°C. |
|
Storage |
Store it under sterile
conditions at -70℃
after receipt. It is recommended that the protein be
aliquoted and be used as soon as possible. Avoid repeated
freeze-thaw cycles. |
|
Gene
Information
|
Gene Name |
CA10 carbonic anhydrase X [ Homo sapiens ] |
|
Synonyms
|
CA10;
carbonic anhydrase X; CARPX; CA-RPX; HUCEP-15; CA-RP X; CARP
X; cerebral protein-15; carbonic anhydrase-related protein
X; carbonic anhydrase-related protein 10; Carbonic
anhydrase-related protein 10; Carbonic anhydrase-related
protein X; Cerebral protein 15 |
|
GeneID |
56934 |
|
mRNA Refseq |
NM_001082533 |
|
Protein
Refseq |
NP_001076002 |
|
MIM |
604642 |
|
UniProt ID |
Q9NS85 |
|
Chromosome
Location |
17q21 |
|
Function |
Molecular
function |
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Download Datasheet:
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Abbr.: 
