|
Cat. No. |
C1S-597H |
|
Product
Overview |
Recombinant
Human C1S was produced in
Human Cells
(HEK293) with a C-terminal his tag. |
|
Description |
Complement
is an integral component of the adaptive and innate immune
systems and represents one of the major effector systems for
the immune responses. The classical complement pathway is
triggered by C1, a complex composed of the binding protein
C1q and two proenzymes, C1r and C1s. Upon binding of IgG to
the head of C1q, C1r undergoes autoactivation and in turn
cleaves and activates C1s. As a highly specific serine
protease, C1s executes the catalytic function of the C1
complex: the cleavage of C4 and C2, and thus instigates a
sequence of activation steps of other components of the
complement system, culminating in the formation of the
membrane attack complex which induces cell lysis. The
single-chain proenzyme C1s is processed into the active form
of disulfide-linked two chains: the A chain contains
multiple domains in the order of
CUB1-EGF-CUB2-CCP1-CCP2-Activation Peptide, while the B
chain contains the serine protease catalytic domain. The
only other protein known to interact with C1s
physiologically is SerpinC1, an inhibitor of serine
protease, which inhibits C1s activity and thus plays a
regulatory role in controlling the function of C1s enzyme. |
|
Molecular
Mass |
The
recombinant human active protease C1s is composed of
disulfide-linked A and B chains. The heterodimer consists of
684 amino acids and has a predicted molecular mass of
76.3kDa. As a result of glycosylation, rhC1s heterodimer
migrates as an approximately 95kDa band in SDS-PAGE under
non-reducing conditions. |
|
Source |
Human Cells
(HEK293). |
|
Predicted N
Terminal |
Glu 16. |
|
Endotoxin |
<1.0 EU per
1μg of the cytokine as determined by the LAL method. |
|
Formulation |
Supplied as
a 0.2μm filtered solution of 100mM Glycine, 10mM NaCl, 50 mM
Tris, pH 7.5. |
|
Reconstitution |
Follow the instructions on the
vial. Centrifuge the vial at 4℃
before opening to recover the entire contents. |
|
Purity |
>97%, as
determined by SDS-PAGE and SEC-HPLC Analysis. |
|
Stability |
Samples are
stable for up to twelve months from date of receipt -70°C. |
|
Storage |
Store at
–70°C; Avoid repeated freeze-thaw cycles. |
|
Gene
Information
|
Gene Name |
C1S complement component 1, s subcomponent [ Homo sapiens ] |
|
Synonyms
|
C1S;
complement component 1, s subcomponent; FLJ44757;
OTTHUMP00000197019; basic proline-rich peptide IB-1;
Complement C1s subcomponent; EC 3.4.21.42; C1 esterase |
|
GeneID |
716 |
|
mRNA Refseq |
NM_001734 |
|
Protein
Refseq |
NP_001725 |
|
MIM |
120580 |
|
UniProt ID |
P09871 |
|
Chromosome
Location |
12p13 |
|
Pathway |
Complement
and coagulation cascades; Systemic lupus erythematosus;
Signaling in Immune system |
|
Function |
calcium ion
binding; peptidase activity; serine-type endopeptidase
activity |
|
PDB rendering based on 1elv.
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Download Datasheet:
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