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Cat. No. |
BCAM-595H
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Product
Overview |
Recombinant Human BCAM
encoding
the extracellular domain of human BCAM (NP_005572.2) (Met 1-
Ala 547) was produced in Human Cells (HEK293). It was fused
with the Fc region of human IgG1 at the C-terminus. |
|
Description |
Basal cell
adhesion molecule (BCAM) and Lutheran blood group
glycoprotein (LU) are two alternatively spliced variants of
a single pre-mRNA. They are type I membrane proteins of the
immunoglobulin superfamily (IgSF) sharing similar structure
characteristics except for the length of cytoplasmic tails.
The mature 597 amino acid BCAM contains an extracellular
domain composing of five Ig-like domains (two V-set and
three C2-set) , a single transmembrane domain, and a 19aa
cytoplasmic domain which lacks the putative Src homology 3
(SH3) binding site compared to the 59aa cytoplasmic domain
present in LU. |
|
Molecular
Mass |
The
recombinant human BCAM/Fc is a disulfide-linked homodimeric
protein. The reduced monomer consists of 754 amino acids and
predicts a molecular mass of 83 KDa. As a result of
glycosylation, the rh BCAM/Fc monomer migrates as
approximately 100-110 KDa band in SDS-PAGE under reducing
conditions. |
|
Source |
Human Cells
(HEK293). |
|
Purity |
> 95%, as
determined by SDS-PAGE. |
|
Endotoxin |
< 1.0 EU
per 1μg cytokine as determined by the LAL method. |
|
Stability |
Samples are stable for up to
twelve months from date of receipt -70℃. |
|
Predicted N
Terminal |
Glu 32. |
|
Formulation |
Supplied as
a 0.2μm filtered solution of PBS, pH7.4. |
|
Reconstitution |
Follow the instructions on the
vial. Centrifuge the vial at 4℃
before opening to recover the entire contents. |
|
Storage |
Store it
under sterile conditions at -70℃. It is recommended that the
protein be aliquoted and be used as soon as possible. Avoid
repeated freeze-thaw cycles. |
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Gene
Information
|
Gene Name |
BCAM basal cell adhesion molecule (Lutheran blood group) [
Homo sapiens ] |
|
Synonyms
|
BCAM; basal
cell adhesion molecule (Lutheran blood group); AU; LU;
CD239; MSK19; Lutheran blood group (Auberger b antigen
included); basal cell adhesion molecule (Lu and Au blood
groups); Basal cell adhesion molecule; Auberger B antigen;
B-CAM cell surface glycoprotein; Lutheran blood group
glycoprotein; Lutheran antigen; F8/G253 antigen;
glycoprotein 95kDa; basal cell adhesion molecule; antigen
identified by monoclonal antibody F8 |
|
GeneID |
4059 |
|
mRNA Refseq |
NM_001013257 |
|
Protein
Refseq |
NP_001013275 |
|
MIM |
612773 |
|
UniProt ID |
P50895 |
|
Chromosome
Location |
19q12-q13 |
|
Function |
laminin
binding; laminin receptor activity |
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Download Datasheet:
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