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Cat. No. |
BACE1-253H
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Product
Overview |
Recombinant
Human BACE1
encoding encoding the extracellular domain (Met 1- Thr 457)
of human BACE1 (NP_036236.1) was expressed in Human Cells
(HEK293) as a secreted chimeric protein. It was fused with
the C-terminal fused Fc region of human IgG1. |
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Description |
As a member
of the peptidase A1 protein family, BACE1 is a type I
integral membrane glycoprotein and aspartic protease that is
found mainly in the Golgi. This protease is responsible for
the proteolytic processing of the amyloid precursor protein
(APP). APP is cleaved to generate a 40 or 42 amino acid-long
amyloid-β peptide, which is the major constituent of amyloid
plaques in the brains of individuals afflicted with
Alzheimers disease (AD), and accordingly, BACE1 is
implicated in the onset and progression of AD. The elevation
of BACE1 levels can be induced by amyloid plaques
surrounding neurons at early stages of pathology before
neuron death occurs, and may drive a positive-feedback loop
in AD. Different isoforms are derived from four transcript
variants. |
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Molecular
Mass |
The
recombinant human BACE1/Fc is a disulfide-linked homodimeric
protein. The reduced monomer consists of 674 amino acids and
has a calculated molecular mass of 75 kDa. As a result of
glycosylation, the rhBACE1/Fc monomer migrates as an
approximately 100-105 kDa protein in SDS-PAGE under reducing
conditions. |
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Source |
Human Cells
(HEK293). |
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Purity |
> 90%, as
determined by SDS-PAGE and SEC-HPLC Analysis. |
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Endotoxin |
< 1.0 EU
per 1μg cytokine as determined by the LAL method. |
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Stability |
Samples are stable for up to
twelve months from date of receipt -70℃. |
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Predicted N
Terminal |
Thr22 and
Glu46 (rare). |
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Formulation |
Supplied as
a 0.2μm filtered solution of PBS, pH7.4. |
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Reconstitution |
Follow the instructions on the
vial. Centrifuge the vial at 4℃
before opening to recover the entire contents. |
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Storage |
Store at
–70°C; Avoid repeated freeze-thaw cycles. |
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Gene
Information
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Gene Name |
BACE1 beta-site APP-cleaving enzyme 1 [ Homo sapiens ] |
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Synonyms
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BACE1;
beta-site APP-cleaving enzyme 1; ASP2; BACE; HSPC104;
FLJ90568; KIAA1149; beta-site APP-cleaving enzyme;
Beta-secretase 1;
EC
3.4.23.46; Beta-site amyloid
precursor protein cleaving enzyme 1; Beta-site APP cleaving
enzyme 1; Membrane-associated aspartic protease 2;
Memapsin-2; Aspartyl protease 2; transmembrane
aspartic proteinase Asp2; APP beta-secretase; Asp 2 |
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GeneID |
23621 |
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mRNA Refseq |
NM_012104 |
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Protein
Refseq |
NP_036236 |
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MIM |
604252 |
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UniProt ID |
P56817 |
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Chromosome
Location |
11q23.2-q23.3 |
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Pathway |
Alzheimer's
disease |
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Function |
protein
binding; aspartic-type endopeptidase activity;
beta-aspartyl-peptidase activity; peptidase activity |
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PDB rendering based on 1fkn.
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Download Datasheet:
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Abbr.: 
