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Cat. No. |
MMP20-162H |
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Product
Overview |
Recombinant
Human matrix metalloproteinase-20 (MMP-20, Enamelysin,
enamel metalloproteinase) catalytic domain cloned from human
cDNA was expressed in E. coli. The enzyme consists of the
catalytic domain of human MMP-20 (residues 113-271. Residues
numbers are based on the unprocessed precursor). Swiss prot.
accession: O60882. MW=17.5kDa calculated. |
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Description |
Matrix
metalloproteinase-20 is an enzyme that in humans is encoded
by the MMP20 gene. Proteins of the matrix metalloproteinase
(MMP) family are involved in the breakdown of extracellular
matrix in normal physiological processes, such as embryonic
development, reproduction, and tissue remodeling, as well as
in disease processes, such as arthritis and metastasis. Most
MMP's are secreted as inactive proproteins which are
activated when cleaved by extracellular proteinases. The
protein encoded by this gene degrades amelogenin, the major
protein component of dental enamel matrix, and so the
protein is thought to play a role in tooth enamel formation.
A mutation in this gene, which alters the normal splice
pattern and results in premature termination of the encoded
protein, has been associated with Amelogenesis imperfecta. |
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Source |
Escherichia
Coli. |
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Purity |
> 95% by
SDS-PAGE. In an SDS-PAGE gel, the enzyme runs as a monomer
(<20kDa). |
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Specific
Activity |
>50U/μg.
1U=100pmol/min at 25ºC using a colorimetric assay with
thiopeptolide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5
(Biomol). |
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Usage |
Study
enzyme kinetics, cleave target substrates and screen of
inhibitors. |
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Supplied As |
0.2mg/ml in 20mM Tris, pH7.2,
10mM CaCl2, 0.1mM ZnCl2, 0.3M NaCl, 0.5M Acetohydroxamic
Acid (AHA). The concentration is calculated by the analysis
of the absorbance at 280nm (e280=31720M-1cm-1
calculated). |
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Characteristics |
Under above
described conditions, to avoid precipitation or protein
dimerization, the product can be concentrated to a maximum
of 100mM.
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Storage |
-80ºC. It
is recommended that thawing and dilution of the enzyme be
done in ice and within as short a time as possible before
start of the assay. After initial defrost, aliquot product
into individual tubes and refreeze at -80ºC. Avoid repeated
freeze/defrost cycles. |
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Gene
Information
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Gene Name |
MMP20 matrix metallopeptidase 20 [ Homo sapiens ] |
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Synonyms
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MMP20;
matrix metallopeptidase 20; Enamel metalloproteinase; matrix
metalloproteinase 20; AI2A2; MMP-20; enamelysin; enamel
metalloproteinase; matrix metalloproteinase 20 (enamelysin);
Matrix metalloproteinase-20; EC 3.4.24.-; Enamelysin |
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GeneID |
9313 |
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mRNA Refseq |
NM_004771 |
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Protein
Refseq |
NP_004762 |
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MIM |
604629 |
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UniProt ID |
O60882 |
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Chromosome
Location |
11q22.3 |
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Function |
calcium ion
binding; metalloendopeptidase activity; peptidase activity;
protein binding; zinc ion binding |
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Download Datasheet:
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