Recombinant Human Matrix Metallopeptidase 7, Catalytic Domain   [ Back  ]                         Price Inquiry

Cat. No.

MMP7-153H

Product Overview

Recombinant Human matrix metalloproteinase-7 (MMP-7, matrilysin, uterine metalloproteinase) cloned from human cDNA was expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-7 (residues 95-267 swissprot accession P09237). MW=19.1kDa.

Description

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Source

E.Coli.

Purity

> 95% by SDS-PAGE. The enzyme was observed as a single band migrating at a molecular weight of (<20 kDa).

Specific Activity

>8U/μg. Activity described as U=100 pmol/min at 25ºC using a colorimetric assay with thiopeptolide Ac-Pro-Leu-Gly-[2-mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.

Usage

Enzyme kinetic studies, cleavage of target substrates and screening of inhibitors.

Supplied As

0.2mg/ml in 20mM Tris, pH 7.2, 10mM CaCl2, 0.1mM ZnCl2, 0.3M NaCl, 0.5M Acetohydroxamic Acid (AHA). The concentration is calculated from the absorbance at 280nm (e280 = 33000 M-1 cm-1).

Note

Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated to a maximum of 0.15 mM.

Storage

-80ºC. The enzyme is stable at -20ºC for at least 1 week. After initial defrost, aliquot enzyme into individual tubes and refreeze at -80ºC. Avoid repeated freeze/defrost cycles.

Gene Information

Gene Name

MMP7 matrix metallopeptidase 7 (matrilysin, uterine) [ Homo sapiens ]

Synonyms

MMP7; matrix metallopeptidase 7 (matrilysin, uterine); MMP-7; MPSL1; PUMP-1; matrix metalloproteinase 7; matrin; uterine matrilysin; matrix metalloproteinase 7 (matrilysin, uterine); EC 3.4.24.23; Matrilysin; Pump-1 protease; Uterine metalloproteinase; Matrix metalloproteinase-7; PUMP1

GeneID

4316

mRNA Refseq

NM_002423

Protein Refseq

NP_002414

MIM

178990

UniProt ID

P09237

Chromosome Location

11q21-q22

Pathway

Wnt signaling pathway

Function

calcium ion binding; metalloendopeptidase activity; peptidase activity; zinc ion binding