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Cat. No. |
FGF2-169H |
|
Product
Overview |
Recombinant
Human FGF-b (FGF-2) produced in Sf9 insect cells is a
single, glycosylated, polypeptide chain containing 155 amino
acids and having a molecular mass of 17353 Dalton.
Recombinant Human FGF-b is purified by proprietary
chromatographic techniques. |
|
Description |
Single-chain polypeptide growth factor that plays a
significant role in the process of wound healing and is a
potent inducer of angiogenesis. It binds to heparin, which
potentiates its biological activity and protects it from
proteolysis. The growth factor is an extremely potent
inducer of DNA synthesis in a variety of cell types from
mesoderm and neuroectoderm lineages, and also has
chemotactic and mitogenic activities. It was originally
named acidic fibroblast growth factor based upon its
chemical properties and to distinguish it from basic
fibroblast growth factor. |
|
Source |
Baculovirus. |
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Physical
Appearance |
Sterile
Filtered liquid formulation. |
|
Formulation |
The sterile
protein solution (0.5mg/ml) contains 20mM Tris pH=7.9, 100mM
KCl, 1mM DTT and 20% glycerol. |
|
Purity |
Greater
than 98.0% as determined by:
(a)
Analysis by RP-HPLC. (b) Anion-exchange FPLC.
(c)
Analysis by reducing and non-reducing SDS-PAGE Silver
Stained gel. |
|
Amino Acid
Sequence |
The
sequence of the first five N-terminal amino acids was
determined and was found to be Ala-Ala-Gly-Ser-Ile. |
|
Dimers And
Aggregates |
Less than
1% as determined by silver-stained SDS-PAGE gel analysis. |
|
Biological
Activity |
Recombinant
Human FGF-b is fully biologically active when compared to
standards. The ED50, calculated by the dose-dependant
proliferation of BAF3 cells expressing FGF receptors
(measured by 3H-thymidine uptake) is <10ng/ml, corresponding
to a specific activity of 2x106 Units/mg. |
|
Protein
Content |
Protein
quantitation was carried out by two independent methods:
1. UV
spectroscopy at 280 nm using the absorbency value of 0.8511
as the extinction coefficient for a 0.1% (1mg/ml) solution.
This value is calculated by the PC GENE computer analysis
program of protein sequences (IntelliGenetics). 2. Analysis
by RP-HPLC, using a calibrated solution of FGF-b as a
Reference Standard. |
|
Stability |
Recombinant
Human FGF-b although stable at 4℃
for 3 weeks, should be stored desiccated below -18℃.
For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
|
Gene
Information
|
Gene Name |
FGF2 fibroblast growth factor 2 (basic) [ Homo sapiens ] |
|
Synonyms
|
FGF2; fibroblast growth factor
2 (basic); BFGF; FGFB; HBGF-2; FGF2; prostatropin;
heparin-binding growth factor 2; basic fibroblast growth
factor bFGF; Heparin-binding growth factor 2 precursor
(HBGF-2);
bFGF; FGF-β; basic fibroblast growth factor |
|
GeneID |
2247 |
|
mRNA Refseq |
NM_002006 |
|
Protein
Refseq |
NP_001997 |
|
MIM |
134920 |
|
UniProt ID |
P09038 |
|
Chromosome
Location |
4q26 |
|
Pathway |
MAPK
signaling pathway; Melanoma; Pathways in cancer; Regulation
of actin cytoskeleton |
|
Function |
growth
factor activity; heparin binding; protein binding;
voltage-gated calcium channel activity |
|
PDB rendering based on 1bas.
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Abbr.: 
