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Cat. No.
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XRCC5-149H
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Product Overview
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Recombinant Human KuP70/P80 (His-tagged) produced in SF9 is a glycosylated, polypeptide chain. The protein has a molecular mass of 43,591 dalton. The protein is purified by proprietary chromatographic techniques.
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Description
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Ku antigen consists of two polypeptides (70 kDa and 80 kDa; the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V(D)J recombination, telomere maintenance, and the regulation of specific gene transcription. Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome (up to 20% of patients) but have also been detectable in about 20 % of patients with primary pulmonary hypertension (as a complication of overlap), in 5-10 % of SLE and MCTD patients, and in up to 20 % of patients with primary Sjögren's syndrome. In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent.
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Source
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Sf9 insect cells.
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Formulation
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Recombinant Human KuP70/P80 is supplied at a of 1mg/ml in16mM HEPES buffer pH-8, 160mM NaCl, and 20% glycerol.
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Purity
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Greater than 90% (SDS-PAGE).
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Application
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Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera.
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Coating Concentration
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0.2-0.6 µg/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
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Immunological Functions
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1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).
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Storage Conditions
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Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
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Gene Information
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Gene Name
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XRCC5 X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) [ Homo sapiens ]
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Synonyms
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X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); KU80; KUB2; Ku86; NFIV; KARP1; KARP-1; FLJ39089; XRCC5; ATP-dependent DNA helicase II; OTTHUMP00000164061; OTTHUMP00000206791; Ku autoantigen, 80kDa; DNA repair protein XRCC5; Ku86 autoantigen related protein 1; X-ray repair, complementing defective, repair in Chinese hamster; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; Ku autoantigen, 80kD); CTC85; CTCBF; EC 3.6.1.-; G22P2; KARP1; Ku80; TLAA; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; Lupus Ku autoantigen protein p86; Nuclear factor IV; Thyroid-lupus autoantigen
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GeneID
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7520
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mRNA Refseq
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NM_021141
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Protein Refseq
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NP_066964
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MIM
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194364
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UniProt ID
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P13010
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Pathway
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Non-homologous end-joining; DNA RepairV; HIV Infection
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Function
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double-stranded telomeric DNA binding; ATP-dependent DNA helicase activity; protein C-terminus binding; ATP binding; DNA binding; helicase activity; hydrolase activity; nucleotide binding; promoter binding
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PDB rendering based on 1jeq.
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Download Datasheet:
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