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Cat. No. |
OSTN-168H
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Product
Overview |
Recombinant Human Osteocrin
(His-tagged) produced in E.coli contains 106 amino acids and has
a molecular mass of 13.6 kDa.
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Description |
Osteocrin is a
recently identified secreted protein expression of which was
only detected in bone, peaking just after birth and decreasing
markedly with age. A 1280-bp mRNA encodes osteocrin producing a
mature protein of 103 amino acids with a molecular mass of 11.4
kDa. In primary osteoblastic cell cultures osteocrin expression
coincided with matrix formation then decreased in very mature
cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3
resulted in a rapid dosedependent down-regulation of osteocrin
expression, suggesting direct regulation. Chronic treatment of
primary cultures with osteocrin-conditioned media inhibited
mineralization and reduced osteocalcin and alkaline phosphatase
expression. These results suggest that osteocrin represents a
novel, unique vitamin D-regulated bone-specific protein that
appears to act as a soluble osteoblast regulator. |
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Source |
Escherichia
Coli. |
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Sequences Of
Amino Acids |
MRGSHHHHHH
GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL
ENDVIETKKK RSFSGFGSPL DRLSAGSVDH KGKQRKVVDH PKRRFGIPMD
RIGRNRLSNS RG. |
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Specificity |
The amino acid
sequence of the recombinant human Osteocrin is 100% homologous
to the amino acid sequence of the human Osteocrin without signal
sequence. |
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Formulation |
Sterile
filtered and lyophilized from 0.5 mg/ml in 5mM TRIS, 25mM NaCl,
pH 7.5. |
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Purification
Method |
One-step
procedure using affinity Ni-NTA chromatography. |
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Purity |
>95 % (SDS-PAGE
analyzed). |
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Reconstitution |
Add 0.2 ml of
dH2O and let the lyophilized pellet dissolve completely.
Storage: Store lyophilized protein at -20°C. Aliquot the product
after reconstitution to avoid repeated freezing/thawing cycles.
Reconstituted protein can be stored at 4°C for a limited period
of time; it does not show any change after two weeks at 4°C. |
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Applications |
Western
blotting, ELISA. |
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Stability/Shelf
Life |
The lyophilized
protein remains stable until the expiry date when stored at
-20°C. |
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Gene
Information
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Gene Name |
OSTN osteocrin [ Homo sapiens ] |
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Synonyms
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OSTN; osteocrin;
MUSCLIN; Musclin |
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GeneID |
344901 |
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mRNA Refseq |
NM_198184 |
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Protein Refseq |
NP_937827 |
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MIM |
610280 |
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UniProt ID |
P61366 |
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Chromosome
Location |
3q29 |
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Function |
hormone
activity |
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Download Datasheet:
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