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Cat. No. |
AGRN-131H
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Product
Overview |
Recombinant
Human agrin antigen (His-tagged) expressed in Sf9 has a
molecular mass of 43,591 Dalton. The isoelectric point of the
antigen is pH 8.9. |
|
Description |
Human α3 chain
of collagen IV; identical with the antigen called "glomerular
basal membrane antigen" (GBM). Recombinant antigen for solid
(ELISA) and fluid phase diagnostic
assays. Binds IgG-type human auto-antibodies. The term
minicollagen designates the removal of most of the epitope-less
triplehelical collagenous region (situated between the
N-terminal 7S domain and the C-terminal noncollagenous NC1
domain), which is a requirement for recombinant production of
this antigen. |
|
Source |
Sf9 insect
cells. |
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Coating
Concentration |
0.12-0.5 μg/ml
(depending on the type of ELISA plate and coating buffer).
Suitable for biotinylation and iodination. |
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Applications |
Standard ELISA
test; Immuno-Dot test with positive/negative sera panels; SDS-PAGE;
Western-Blot. |
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Caution |
It has been
reported that the immunodominant epitope of GBM is a cryptic
epitope that is not easily accessible to the corresponding
autoantibodies. It is necessary to treat the protein under
nonreducing conditions with a denaturant such as urea to unmask
the epitopes (see Hellmark et al. in Autoantibodies, Peter, J.B.
and Shoenfeld, Y., eds., Elsevier B.V., 1996, pp 291-298). |
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Storage &
Stability |
Recommendations
for storage buffer: ion strength 100mM, neutral to slightly
alkaline pH and 20 % glycerol as cryoprotective agent. Storage
temperature: –70° to –80° C. Please prevent freeze-thaw cycles. |
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Gene
Information
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Gene Name |
AGRN agrin [ Homo sapiens ] |
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Synonyms
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AGRN; agrin;
FLJ45064; agrin proteoglycan; Agrin;
AGRIN |
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GeneID |
375790 |
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mRNA Refseq |
NM_198576 |
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Protein Refseq |
NP_940978 |
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MIM |
103320 |
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UniProt ID |
O00468 |
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Chromosome
Location |
1p36.33 |
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Pathway |
ECM-receptor
interaction; Axon guidance |
|
Function |
acetylcholine
receptor regulator activity; laminin binding; structural
constituent of cytoskeleton |
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Abbr.: 
