Recombinant Human VAMP2   [ Back  ]                         Price Inquiry

Cat. No.

VAMP2-216H

Product Overview

Recombinant Human VAMP2 produced in E.Coli is a single, non-glycosylated polypeptide chain. The protein contains 126 amino acids and has a molecular mass of 13.8 kDa.

Description

Synaptobrevin 2 which is an 18 kDa integral membrane protein localized to the cytoplasmic surface of synaptic vesicle, consists of a proline-rich N-terminal region, a highly conserved hydrophilic domain, followed by a transmembrane anchor and a C-terminal. Synaptobrevin 2 is predominantly expressed in Langerhans islets and glomerular cells. The N-terminal domain of the protein (residues 1-89) forms a specific SNARE complex with the target membrane-associated t- or Q-SNAREs syntaxin 1 and SNAP-25.

Source

Escherichia Coli.

Amino Acid Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMSA TAATAPPAAP AGEGGPPAPP PNLTSNRRLQ QTQAQVDEVV DIMRVNVDKV  LERDQKLSEL  DDRADALQAG  ASQFETSAAK.

Physical Appearance

Sterile Filtered colorless solution.

Purity

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Formulation

The protein solution (1mg/ml) contains 1X PBS and 1mM EDTA.

Stability

Synaptobrevin although stable 14℃ for 4 weeks, should be stored desiccated below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

Gene Name

VAMP2 vesicle-associated membrane protein 2 (synaptobrevin 2) [ Homo sapiens ]

Synonyms

GeneID

6844

mRNA Refseq

NM_014232

Protein Refseq

NP_055047

UniProt ID

P63027

Chromosome Location

17p13.1

MIM

185881

Pathway

SNARE interactions in vesicular transport; Botulinum neurotoxicity; Diabetes pathways; Integration of energy metabolism; Membrane Trafficking; Synaptic Transmission

Function

protein binding