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Cat. No.
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YWHAZ-104H
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Product Overview
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Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (245 aa, 32kDa).
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Description
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The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, β, γ, ε, σ, ζ, τ and η that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity.
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Source
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E.coli.
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Formulation
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Liquid in Phosphate Buffered Saline pH7.4.
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Molecular Weight
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32 kDa (245 aa).
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Purity
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> 95% by SDS – PAGE.
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Concentration
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1 mg/ml (determined by Bradford assay).
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Sequence
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MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN
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Storage
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Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
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Gene Information
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Gene Name
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YWHAZ
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Synonyms
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YWHAZ; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; KCIP-1; MGC111427; MGC126532; MGC138156; phospholipase A2; 14-3-3 zeta; 14-3-3 protein/cytosolic phospholipase A2; Protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; OTTHUMP00000165851; OTTHUMP00000165852; OTTHUMP00000165854; OTTHUMP00000165858; OTTHUMP00000165859; OTTHUMP00000165860; YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide.
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GeneID
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7534
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mRNA Refseq
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NM_001135699.1
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Protein Refseq
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NP_001129171.1
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MIM
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601288
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UniProt ID
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P63104
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Chromosome Location
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8q23.1
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Pathway
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Cell cycle; Pathogenic Escherichia coli infection - EHEC; Pathogenic Escherichia coli infection - EPEC.
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Function
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protein domain specific binding; transcription factor binding.
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