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Cat. No. |
GAPDH-115H |
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Product
Overview |
Recombinant
GAPDH protein was expressed in E.coli and purified by using
conventional chromatography techniques, 36kDa (335aa). |
|
Description |
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic
enzyme commonly known to be involved in glycolysis. The enzyme
exists as a tetramer composed of 36-kDa subunits and has various
intracellular functions. GAPDH catalyzes the reversible
reduction of 1,3-bisphosphoglycerate to glyceraldehyde
3-phosphophate in the presence of NADPH. Besides functioning as
a glycolytic enzyme in cytoplasm, evidence suggests that
mammalian GAPDH is also involved in a great number of
intracellular processes such as membrane fusion, microtubule
bundling, phosphotransferase activity, nuclear RNA export, DNA
replication and DNA repair. |
|
Sequences of
amino acids |
MGKVKVGVNG
FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV
KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA
HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL
AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS
TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK
VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL
ISWYDNEFGY SNRVVDLMAH MASKE |
|
Formulation |
Liquid. In 20
mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 1 mM EDTA,
20% glycerol |
|
Purity |
> 95% by SDS -
PAGE |
|
Concentration |
1 mg/ml
(determined by Bradford assay) |
|
Storage |
Can be stored
at +4°C short term (1-2 weeks). For long term storage, aliquot
and store at -20°C or -70°C. Avoid repeated freezing and thawing
cycles. |
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Gene
Information
|
Gene Name |
GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens ]
|
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Synonyms
|
glyceraldehyde-3-phosphate
dehydrogenase; G3PD; GAPD; MGC88685; GAPDH;
glyceraldehyde-3-phosphate dehydrogenase; OTTHUMP00000174431; EC
1.2.1.12; OTTHUMP00000174432; aging-associated gene 9 protein;
glyceraldehyde 3-phosphate dehydrogenase |
|
GeneID |
2597 |
|
mRNA Refseq |
NM_002046 |
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Protein Refseq |
NP_002037 |
|
MIM |
138400 |
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UniProt ID |
P04406 |
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Chromosome
Location |
12p13 |
|
Pathway |
Alzheimer's
disease; Dentatorubropallidoluysian atrophy (DRPLA); Glycolysis
/ Gluconeogenesis; Huntington's disease; Neurodegenerative
Disorders; Metabolism of carbohydrates |
|
Function |
NAD or NADH
binding; glyceraldehyde-3-phosphate dehydrogenase
(phosphorylating) activity; oxidoreductase activity; protein
binding |
|
PDB rendering based on 3GPD.
Available structures:
1j0x,
1u8f,
1znq
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Download Datasheet:
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