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Cat. No. |
DDC-141H
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Product
Overview |
Recombinant
human Dopa decarboxylase protein, fused to His-tag at
N-terminus, was expressed in E.coli and purified by using
conventional chromatography, 56.4 kDa (503aa). |
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Description |
Dopa
decarboxylase, also known as DDC, is a homodimeric, pyridoxal
phosphate dependent enzyme. Dopa decarboxylase is a protein
implicated in 2 metabolic pathways, synthesizing 2 important
neurotransmitters: dopamine and serotonin which both play key
roles in many clinical disorders, including Parkinson’s disease.
Dopa decarboxylase is found in different areas of the brain and
is particularly abundant in basal ganglia. |
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Sequences of
amino acids |
MGSSHHHHHH
SSGLVPRGSH TRSMNASEFR RRGKEMVDYV ANYMEGIEGR QVYPDVEPGY
LRPLIPAAAP QEPDTFEDII NDVEKIIMPG VTHWHSPYFF AYFPTASSYP
AMLADMLCGA IGCIGFSWAA SPACTELETV MMDWLGKMLE LPKAFLNEKA
GEGGGVIQGS ASEATLVALL AARTKVIHRL QAASPELTQA AIMEKLVAYS
SDQAHSSVER AGLIGGVKLK AIPSDGNFAM RASALQEALE RDKAAGLIPF
FMVATLGTTT CCSFDNLLEV GPICNKEDIW LHVDAAYAGS AFICPEFRHL
LNGVEFADSF NFNPHKWLLV NFDCSAMWVK KRTDLTGAFR LDPTYLKHSH
QDSGLITDYR HWQIPLGRRF RSLKMWFVFR MYGVKGLQAY IRKHVQLSHE
FESLVRQDPR FEICVEVILG LVCFRLKGSN KVNEALLQRI NSAKKIHLVP
CHLRDKFVLR FAICSRTVES AHVQRAWEHI KELAADVLRA ERE |
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Formulation |
Liquid. In 20
mM Tris-HCl buffer (pH 8.0) containing 2mM DTT 10% glycerol. |
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Purity |
> 95% by SDS -
PAGE |
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Concentration |
1 mg/ml
(determined by Bradford assay) |
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Storage |
Can be stored
at +4°C short term (1-2 weeks). For long term storage, aliquot
and store at -20°C or -70°C. Avoid repeated freezing and thawing
cycles. |
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Gene
Information
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Gene Name |
dopa decarboxylase [ Homo sapiens ] |
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Synonyms
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DDC; dopa
decarboxylase (aromatic L-amino acid decarboxylase); AADC; L-Dopa
decarboxylase; aromatic L-amino acid decarboxylase; EC
4.1.1.28;
DOPA decarboxylase |
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GeneID |
1644 |
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mRNA Refseq |
NM_ 000790 |
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Protein Refseq |
NP_ 000781 |
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MIM |
107930 |
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UniProt ID |
P20711 |
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Chromosome
Location |
7p12.2
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Pathway |
Alkaloid
biosynthesis I; Histidine metabolism; Phenylalanine metabolism;
Tryptophan metabolism; Tyrosine metabolism; Synaptic
Transmission |
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Function |
aromatic-L-amino-acid decarboxylase activity; lyase activity;
protein binding; pyridoxal phosphate binding |
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Ribbon diagram of a domestic pig DOPA decarboxylase
dimer. From PDB 1JS3
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Download Datasheet:
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Abbr.: 
