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Cat. No. |
AK3L1-102H |
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Product
Overview |
Recombinant
human AK3, fused to His-tag at N-terminus, was expressed in
E.coli and purified by using conventional chromatography
techniques (259 aa, 29.3 kDa). |
|
Description |
Adenylate
kinase (AK; adenosine triphosphate-adenosine monophosphate
[ATP-AMP] phospho-transferase, EC 2.7.4.3) is a ubiquitous
monomeric enzyme involved energy metabolism of prokaryotic and
eukaryotic cells. Five isozymes of adenylate kinase have been
identified in vertebrates. AK1 is present in the cytosol of
skeletal muscle, brain, and erythrocyte, while AK2 is localized
in the intermembrane space of mitochondria of liver, kidney,
spleen and heart. AK3, called GTP: AMP phosphotransferase,
exists in the mitochondrial matrix of liver and heart. These
isozymes contribute to homeostasis of the adenine nucleotide
composition in the cell.
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|
Source |
E. coli. |
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Formulation
|
Liquid. In 20
mM Tris-HCl buffer (pH 8.0) containing 2 mM DTT, 20% glycerol. |
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Molecular
Weight |
29.3 kDa (259
aa). |
|
Purity |
> 90% by SDS –
PAGE. |
|
Concentration |
1 mg/ml
(determined by Bradford assay). |
|
Sequence |
MRGSHHHHHH
GMASMTGGQQ MGRDLYDDDD KDRWGSMASK LLRAVILGPP GSGKGTVSQR
IAQNFGLQHL SSGHFLRENI KASTEVGEMA KQYIEKSLLV PDHVITRLMM
SELENRRGQH WLLDGFPRTL GQAEALDKIC EVDLVISLNI PFETLKDRLS
RRWIHPPSGR VYNLDFNPPH VHGIDDVTGE PLVQQEDDKP EAVAARLRQY
KDVAKPVIEL YKSRGVLHQF SGTETNKIWP YVYTLFSNKI TPIQSKEAY |
|
Storage |
Can be stored
at +4°C short term (1-2 weeks). For long term storage, aliquot
and store at -20°C or -70°C. Avoid repeated freezing and thawing
cycles. |
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Gene
Information |
Gene Name |
AK3L1 |
|
Synonyms |
AK3L1;
adenylate kinase 3-like 1; AK3L1 adenylate kinase 3-like 1; AK3;
AK4; MGC166959; EC 2.7.4.3; OTTHUMP00000010594; ATP-AMP
transphosphorylase; GTP:AMP phosphotransferase; Adenylate kinase
3-like; adenylate kinase 3; mitochondrial adenylate kinase-3;
nucleoside-triphosphate-adenylate kinase. |
|
GeneID |
205 |
|
mRNA Refseq |
NM_001005353.1 |
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Protein Refseq |
NP_001005353.1 |
|
MIM |
103030 |
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UniProt ID |
P27144 |
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Chromosome
Location |
1p31.3 |
|
Pathway |
Purine
metabolism |
|
Function |
ATP binding;
GTP binding; adenylate kinase activity; nucleotide binding;
transferase activity |
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PDB rendering based on 2ar7.
Available structures:
2ar7,
2bbw.
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Download Datasheet::
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