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Cat. No.
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ATPS-78Y
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Product Overview
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Recombinant Yeast Adenosine 5" Triphosphate Sulfurylase produced in E.Coli is a non-glycosylated, polypeptide chain which catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5´-phosphosulfate (APS) and pyrophosphate (PPi). The reaction is reversible: ATP is formed from APS and PPi. Adenosine 5 Triphosphate Sulfurylase is purified by proprietary chromatographic techniques.
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Introduction
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ATP sulphurylase synthesizes adenosine 5’-sul-phatophosphate from ATP and inorganic SO42-. This is the first reaction of a two step sequence in the formation of “active sulphate”, adenosine 3’-phosphate5’-sulphatophosphate, which is a sulphate donor for a wide variety of compounds and is also involved in the reduction of sulphate.
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Synonyms
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EC 2.7.7.4; SAT; ATPSK1; PAPSS; PAPSS 1; SK1; ATPS; ATP-sulfurylase; Sulfurylase kinase 1; PAPS Sulfurylase; synthetase 1; Sulfate adenylyltransferase; Sulfate adenylate transferase; Methionine-requiring protein 3; 3'-phosphoadenosine 5'-phosphosulfate synthase 1; 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1; adenosine-5'-triphosphate sulfurylase; adenosinetriphosphate sulfurylase; adenylylsulfate pyrophosphorylase; ATP sulfurylase; ATP-sulfurylase; sulfurylase; ATP:sulfate adenylyltransferase.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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Greater than 95.0% as determined by SDS-PAGE.
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Formulation
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The protein was lyophilized after dialysis against 10 mM NaP, 100 mM NaCl, 10 mM Lactose, 1% PEG, pH 7.5.
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Solubility
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It is recommended to reconstitute the lyophilized ATP-sulfurylase in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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Biological Activity
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12 Units/mg.
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Stability
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Lyophilized Sulfate adenylate transferase although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ATPS should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
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Download Datasheet:
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