Recombinant Mouse Resistin, Flag-tagged   [ Back  ]                         Price Inquiry

Cat. No.

Retn-68M

Product Overview

Recombinant Mouse Resistin is manufactured with signal sequence of phage fd (21aa) and C-terminal fusion of flagTag (10aa). Resistin Mouse Recombinant Flag-Tagged Fusion Protein is 13.7 kDa protein containing 93 amino acid residues of the Resistin Mouse and 31 additional amino acid residues - signal sequence of phage fd, flagTag (underlined). MKKLLFAIPL VVPFYSHSTM ASMPLCPIDE AIDKKIKQDF NSLFPNAIKN IGLNCWTVSS RGKLASCPEG TAVLSCSCGS ACGSWDIREE KVCHCQCARI DWTAARCCKL QVASLEDYKD DDDK.

Description

Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake. They have also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severly suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.

Source

Escherichia Coli.

Specificity

The amino acid sequence of the Recombinant Mouse Resistin is 100% homologous to the amino acid sequence of the Resistin Mouse.

Purification Method

Two-step procedure using size exclusion chromatography before and after refolding.

Purity

>95% (SDS-PAGE analyzed).

Formulation

Sterile filtered and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4.

Solubility

Add 0.2 ml of 0.1 M Acetate buffer pH4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/ml. In higher concentrations the solubility of this antigen is limited.

Applications

Western blotting, ELISA.

Stability

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored.

Gene Information

Gene Name

Retn resistin [ Mus musculus ]

Synonyms

Retn; resistin; ADSF; Rstn; Xcp4; Fizz3; found in inflammatory zone 3; adipocyte-specific secretory factor; rstn

GeneID

57264

mRNA Refseq

NM_022984

Protein Refseq

NP_075360

UniProt ID

Q99P87

Chromosome Location

8 A1; 8 0.37 cM

Function

hormone activity