|
Cat. No. |
NAMPT-125H |
|
Product
Overview |
Recombinant
Human Visfatin produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 348 amino acids and having 11
N-terminal flag tag The total molecular mass is 39573 Dalton.
The amino acid sequence of the recombinant human Visfatin is
100% homologous to the amino acid sequence Pro27-Glu363 of the
human Visfatin. The Visfatin is purified by Flag-affinity
chromatography. |
|
Description |
Visfatin, also
known as pre-B cell colony-enhancing factor (PBEF), is a
cytokine that is highly expressed in visceral fat and was
originally isolated as a secreted factor that synergizes with
IL-7 and stem cell factors to promote the growth of B cell
precursors. Visfatin homologs have been identified in carp,
invertebrate mollusks, and bacteria, as well as in vertebrates.
Visfatin exerts insulin-mimetic effects that are dose-dependent
and quantitatively similar to those of insulin in stimulating
muscle and adipocyte glucose transport, and in inhibiting
hepatocyte glucose production. Intravenous injection of
recombinant visfatin in mice decreased plasma glucose in a
dose-dependent fashion. In keeping with its insulin-mimetic
effects, visfatin was as effective as insulin in reducing
hyperglycemia in insulin-deficient diabetic mice. Visfatin was
also found to be bound to and activate insulin receptor, causing
receptor phosphorylation and the activation of downstream
signaling molecules. |
|
Source |
Escherichia
Coli. |
|
Amino acid
sequence |
MDYKDDDDKA
SPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV
TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI
PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY
LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA
LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS
VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL
EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQE. |
|
Physical
Appearance |
Sterile
Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
Greater than
95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by
SDS-PAGE. |
|
Formulation |
Filtered (0.4
µm) and lyophilized from 0.5 mg/ml in 20 mM Tris, 20 mM NaCl, pH
7.5. |
|
Applications |
ELISA, Western
Blot. |
|
Solubility |
It is
recommended to reconstitute the lyophilized Visfatin in sterile
18 MΩ-cm H2O not less than 100 µg/ml, which can then be further
diluted to other aqueous solutions. |
|
Stability |
Lyophilized
Visfatin although stable at room temperature for 3 weeks, should
be stored desiccated below -18℃. Upon reconstitution Visfatin
should be stored at 4℃ between 2-7 days and for future use
below -18℃. For long term storage it is recommended to add a
carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw
cycles. |
|
Gene
Information
|
Gene Name |
NAMPT nicotinamide phosphoribosyltransferase [ Homo sapiens ] |
|
Synonyms |
NAMPT;
nicotinamide phosphoribosyltransferase; VF; PBEF; PBEF1;
VISFATIN; MGC117256; DKFZp666B131; 1110035O14Rik; NAmPRTase;
pre-B cell-enhancing factor; pre-B-cell colony enhancing factor
1; EC 2.4.2.12 ; Nampt; Visfatin |
|
GeneID |
10135 |
|
mRNA Refseq |
NM_005746 |
|
Protein Refseq |
NP_005737 |
|
MIM |
608764 |
|
UniProt ID |
P43490 |
|
Chromosome
Location |
7q22.3 |
|
Pathway |
Nicotinate and
nicotinamide metabolism |
|
Function |
cytokine
activity; nicotinamide phosphoribosyltransferase activity;
nicotinamide phosphoribosyltransferase activity; transferase
activity, transferring glycosyl groups |
|
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Abbr.: 
