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Cat. No. |
CCL11-26H
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Product
Overview |
Recombinant
Human Eotaxin produced in E.Coli is a single, non-glycosylated
polypeptide chain containing 74 amino acids and having a
molecular mass of 8345.9 Dalton. The CCL11 is purified by
proprietary chromatographic techniques. |
|
Description |
Chemokine (C-C
motif) ligand 11 (CCL11) is a small cytokine belonging to the CC
chemokine family that is also known as eotaxin. CCL11
selectively recruits eosinophils by inducing their chemotaxis,
and therefore, is implicated in allergic responses. The effects
of CCL11 are mediated by its binding to a G-protein-linked
receptor known as a chemokine receptor. Chemokine receptors for
which CCL11 is a ligand include CCR2, CCR3 and CCR5. The gene
for human CCL11 (scya11) is encoded on three exons and is
located on chromosome 17. |
|
Source |
Escherichia
Coli. |
|
Amino Acid
Sequence |
The sequence of
the first five N-terminal amino acids was determined and was
found to be Gly-Pro-Ala-Ser-Val. |
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Physical
Appearance |
Sterile
Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
Greater than
98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by
SDS-PAGE. |
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Formulation |
Lyophilized
from a concentrated (1 mg/ml) solution in water containing no
additives. |
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Solubility |
It is
recommended to reconstitute the lyophilized Eotaxin Human
Recombinant in sterile 18MΩ-cm H2O not less than 100 µg/ml,
which can then be further diluted to other aqueous solutions. |
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Biological
Activity |
The activity is
determined by the chemoattract of human PBE (peripheral blood
eosinophils) at a concentration between 0.1-10 ng/ml. |
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Protein Content |
Protein
quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of
1.013 as the extinction coefficient for a 0.1% (1 mg/ml)
solution. This value is calculated by the PC GENE computer
analysis program of protein sequences (IntelliGenetics). 2.
Analysis by RP-HPLC, using a standard solution of Eotaxin as a
Reference Standard. |
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Storage |
Lyophilized
Eotaxin although stable at room temperature for 3 weeks, should
be stored desiccated below -18℃.
Upon reconstitution CCL11 should be stored at 4℃
between 2-7 days and for future use below -18℃.
For long term storage it is recommended to add a carrier protein
(0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
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Gene
Information
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Gene Name |
CCL11 chemokine (C-C motif) ligand 11 [ Homo sapiens ] |
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Synonyms
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CCL11;
chemokine (C-C motif) ligand 11; C-C motif chemokine 11;
eotaxin; eotaxin-1; Eosinophil chemotactic protein; MGC22554;
Small-inducible cytokine A11; small inducible cytokine A11;
SCYA11; small inducible cytokine subfamily A (Cys-Cys), member
11 (eotaxin) |
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GeneID |
6356 |
|
mRNA Refseq |
NM_002986 |
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Protein Refseq |
NP_002977
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MIM |
601156 |
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UniProt ID |
P51671 |
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Chromosome
Location |
17q21.1-q21.2
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Pathway |
Asthma;
Chemokine signaling pathway; Cytokine-cytokine receptor
interaction |
|
Function |
chemokine
activity |
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Abbr.: 
