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Cat. No.
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CRP21P
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Product Overview
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Noggin Human Recombinant produced in E.Coli is a non-glycosylated, non-disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains, having a total molecular mass of approximately 46.2 kDa (each chain 23.1 kDa).Noggin is purified by proprietary chromatographic techniques.
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Description
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Noggin is a polypeptide that inhibits TGF-β signal transduction by binding to TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin plays a key role in neural induction by inhibiting BMP4, along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development, joint formation, and neural tube fusion.
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Purity
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Greater than 95.0% as determined by SDS-PAGE.
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Source
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Escherichia Coli.
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Amino acid sequence
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MQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGH
YDPGFMATSPPEDRPGGGGGAAGGAEDLAELDQLLRQRPSGAMPS
EIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDL
GSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQR
RGQRCGWIPIQYPIISECKCSC
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Biological Activity
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The ED50 was determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells. The expected ED50 for this effect is 0.05-0.08 μg/ml of Noggin.
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Formulation
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Lyophilized from a 0.2μm filtered solution in 30% CH3CN, 0.1% TFA.
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Protein content
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Protein quantitation was carried out by two independent methods: UV spectroscopy at 280 nm using the absorbency value of 1.76 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics); Analysis by RP-HPLC, using a standard solution of Noggin as a Reference Standard.
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Solubility
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It is recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.
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Stability
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Lyophilized Noggin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Noggin should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
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Gene Information
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Gene Name
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NOG noggin [ Homo sapiens ]
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Synonyms
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SYM1; SYNS1; NOG; noggin; symphalangism 1 (proximal); synostoses (multiple) syndrome 1
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GeneID
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9241
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mRNA Refseq
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NM_005450
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Protein Refseq
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NP_005441
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MIM
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602991
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UniProt ID
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Q13253
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Chromosome Location
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17q21-q22
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Function
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cytokine binding; protein homodimerization activity
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PDB rendering based on 1m4u. Available structures: 1m4u
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Download Datasheet:
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