|
Cat. No. |
CCL3-40H |
|
Product
Overview |
Macrophage
Inflammatory Protein-1 alpha Human Recombinant produced in
E.Coli is a single, non-glycosylated, polypeptide chain
containing 70 amino acids and having a molecular mass of 7820
Dalton. |
|
Description |
Macrophage
Inflammatory Proteins (MIP) belong to the family of chemotactic
cytokines known as chemokines. In humans, there are two major
forms, MIP-1α and MIP-1β that are now officially named CCL3 and
CCL4 respectively. Both are major factors produced by
macrophages after they are stimulated with bacterial endotoxins.
They activate human granulocytes(neutrophils, eosinophilsand
basophils) which can lead to acute neutrophilic inflammation.
They also induce the synthesis and release of other
pro-inflammatory cytokines such as interleukin 1 (IL-1), IL-6
and TNF-α from fibroblasts and macrophages. The genes for CCL3
and CCL4 are both located on human chromosome 17. |
|
Source |
Escherichia
Coli. |
|
Physical
Appearance |
Sterile
Filtered White lyophilized (freeze-dried) powder. |
|
Purity |
Greater than
98.0% as determined by: (a) Analysis by RP-HPLC; (b) Analysis by
SDS-PAGE. |
|
Formulation |
The protein was
lyophilized from 0.55mg/ml solution containing no additives. |
|
Solubility |
It is
recommended to reconstitute the lyophilized Macrophage
Inflammatory Protein-1a in sterile 18MΩ-cm H2O not less than
100µg/ml, which can then be further diluted to other aqueous
solutions. |
|
Specific
Activity |
The Activity is
calculated by the ability of chemo-attraction of Human monocytes
using 1-10ng/ml. |
|
Protein content |
Protein
quantitation was carried out by two independent methods: 1. UV
spectroscopy at 280 nm using the absorbency value of 0.9 as the
extinction coefficient for a 0.1% (1mg/ml) solution. This value
is calculated by the PC GENE computer analysis program of
protein sequences (IntelliGenetics); 2. Analysis by RP-HPLC,
using a calibrated solution of MIP-1a as a Reference Standard. |
|
Stability |
Lyophilized
MIP-1a although stable at room temperature for 3 weeks, should
be stored desiccated below -18°C. Upon reconstitution CCL3
should be stored at 4°C between 2-7days and for future use below
-18°C. For long term storage it is recommended to add a carrier
protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
|
Gene
Information
|
Gene Name |
CCL3 chemokine (C-C motif) ligand 3 [ Homo sapiens ] |
|
Synonyms
|
chemokine (C-C
motif) ligand 3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha;
CCL3; LD78
alpha beta; small inducible cytokine A3 (homologous to mouse
Mip-1a); SIS-beta; G0/G1 switch regulatory protein 19-1; G0S19-1
protein; LD78 alpha beta; Macrophage inflammatory protein
1-alpha; PAT 464.1; Small-inducible cytokine A3; Tonsillar
lymphocyte LD78 alpha protein; C-C motif chemokine 3;
Small-inducible cytokine A3; Short name=MIP-1-alpha; Tonsillar
lymphocyte LD78 alpha protein; G0/G1 switch regulatory protein
19-1; Short name=G0S19-1 protein; SIS-beta; PAT 464.1 |
|
GeneID |
6348 |
|
mRNA Refseq |
NM_002983 |
|
Protein Refseq |
NP_002974 |
|
MIM |
182283 |
|
UniProt ID |
P10147 |
|
Chromosome
Location |
17q11-q21 |
|
Pathway |
Cytokine-cytokine receptor Toll-like receptor signaling pathway;
Chemokine signaling pathway; Cytokine-cytokine receptor
interaction |
|
Function |
chemoattractant
activity; chemokine activity; signal transducer activity |
|
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Abbr.: 
