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Cat. No.
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CCL14-23H
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Product Overview
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Recombinant Human HCC-1 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 72 amino acids and having a molecular mass of 8411 Dalton. The HCC-1 is purified by proprietary chromatographic techniques.
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Description
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Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is procesed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL13 activates monocytes, but does not induce their chemotaxis. Human CCL13 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.
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Source
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Escherichia Coli.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Purity
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Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
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Formulation
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The CCL14 protein was lyophilized with 20 mM PBS pH 7.4 and 150 mM NaCl.
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Solubility
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It is recommended to reconstitute the lyophilized HCC-1 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
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Specific Activity
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The Biological activity is calculated by its ability to chemoattract Human monocytes at 5-20 ng/ml.
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Stability
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Lyophilized HCC1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL14 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
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Gene Information
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Gene Name
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CCL14 chemokine (C-C motif) ligand 14 [ Homo sapiens ]
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Synonyms
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SCYA14; SY14; SCYL2; HCC-1; HCC-3; NCC-2; NCC2; CKb1; MCIF; CC-1; CC-3; CCL14; FLJ16015; HCC-1(1-74); HCC-1/HCC-3; chemokine (C-C motif) ligand 14; chemokine CC-1; chemokine CC-3; Chemokine CC-1/CC-3; OTTHUMP00000176860; Small-inducible cytokine A14; small inducible cytokine subfamily A (Cys-Cys), member 14
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GeneID
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6358
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mRNA Refseq
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NM_004166
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Protein Refseq
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NP_004157
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MIM
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601392
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UniProt ID
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Q16627
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Chromosome Location
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17q11.2
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Pathways
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Cytokine-cytokine receptor interaction
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Function
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chemokine activity; protein binding; signal transducer activity
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