Recombinant Murine Chemokine (C-X-C Motif) Ligand 12(SDF1b)   [ Back  ]                         Price Inquiry

Cat. No.

CXCL12-21M

Production Description

SDF-1α and SDF-1β, members of the chemokine α subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1α and SDF-1β cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1α and SDF-1β are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1β and absent from SDF-1α.

Source

Escherichia coli.

Molecular Weight

8.5 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids.

Quantity

2ug/10ug/1000μg

Purity

>97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. Determined by its ability to chemoattract human monocytes using a concentration range of 50.0-100.0 ng/ml.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.

Endotoxin

Less than 1EU/μg of rMu SDF-1 beta /CXCL12 as determined by LAL method.

AA Sequence

KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNKRL KM

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

Storage

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.

Usage

This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.

Gene Information

Gene Name

Cxcl12 chemokine (C-X-C motif) ligand 12 [ Mus musculus ]

Synonyms

CXCL12; PBSF; SCYB12; SDF-1a; SDF-1b; SDF1; SDF1A; SDF1B; TLSF-a; TLSF-b; TPAR1; chemokine (C-X-C motif) ligand 12; Sdf1; TLSF; SDF-1; Sdf1a; Sdf1b; TPAR1; Scyb12; AI174028; PBSF/SDF-1; Cxcl12; stromal cell derived factor 1; stromal cell derived factor 1;OTTMUSP00000026112; OTTMUSP00000026113; OTTMUSP00000026114; pre-B-cell growth-stimulating factor; OTTHUMP00000019491; hIRH; C-X-C motif chemokine 12; chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); stromal cell-derived factor 1; stromal cell-derived factor 1 delta; stromal cell-derived factor 1 gamma; stromal cell-derived factor 1a.

GeneID

20315

mRNA Refseq

NM_001012477

Protein Refseq

NP_001012495

UniProt ID

P40224

Chromosome Location

6 F1; 6 53.0 cM

Pathways

Axon guidance; Cytokine-cytokine receptor interaction; Leukocyte transendothelial migration.

Function

Chemokine activity; cytokine activity; growth factor activity.