Recombinant Human Interleukin-10   [ Back  ]                         Price Inquiry

Cat. No.

IL10-16H

Product Overview

Recombinant Human IL-10 produced in E. coli is a single, non-glycosylated polypeptide chain containing 160 amino acids and having a molecular mass of 18648 Dalton.

Description

In humans IL-10 is produced by activated CD8 (+) peripheral blood T-cells, by T-helper CD4 (+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cell lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secrete large quantities of IL-10 into the conditioned medium. The synthesis of IL-10 by monocytes is inhibited by IL-4 and IL-10.

Purity

>98% as determined by SDS-PAGE , RP-HPLC and FPLC.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution containing 60mM PBS pH-6.5 and 100mM NaCl.

Specific Activity

The ED50 as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 10⁶ IU/mg.

Endotoxin

Less than 0.1 ng/µg (IEU/µg) of rHuIL-10.

Reconstitution

It is recommended to reconstitute the lyophilized rHuIL-10 in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage

Lyophilized rHuIL-10 although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuIL-10 should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

IL10

Synonyms

CSIF; IL10A; MGC1264 50; MGC126451; TGIF; B-TCGF; IL-10; Cytokine synthesis inhibitory factor;Interleukin-10 precursor; IL10; interleukin 10

GeneID

3586

mRNA Refseq

NM_000572

Protein Refseq

NP_000563

MIM

124092

UniProt ID

P22301

Chromosome Location

1q31-q32

Pathway

Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; T cell receptor signaling pathway

Function

cytokine activity; interleukin-10 receptor binding; protein binding