Recombinant Human Interferon-alpha 2b   [ Back  ]                         Price Inquiry

Cat. No.

IFNA2-03H

Product Overview

Recombinant Human Interferon-alpha 2b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 166 amino acids and having a molecular mass of 19400 Dalton. The Interferon-alpha 2b gene was obtained from human leukocytes.

Description

At least 23 different variants of IFN-alpha are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.

Purity

>95% as determined by SEC-HPLC and SDS-PAGE

Formulation

Lyophilized from a (1mg/ml) solution in containing 8.8mg sodium chloride, 0.4mg sodium acetate and 1.0mg acetate acid buffer.

Specific Activity

The specific activity as determined in a viral resistance assay using VSV- WISH cells was found to be greater than 1.0 x 10⁸ IU/ mg.

Endotoxin

Less than 0.3ng/µg (0.3IEU/µg) determined by LAL test.

Reconstitution

It is recommended to reconstitute the lyophilized rHuIFN-alpha2b in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Storage

Lyophilized rHuIFN-alpha 2b although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution rHuIFN-alpha 2b should be stored at 4^oC between 2-7 days and for future use below -18^oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Aliquot to avoid repeated freeze-thaw cycles.

Gene Information

Gene Name

IFNA2

Synonyms

IFNA; INFA2; MGC125764; MGC125765; Leukocyte interferon; B cell interferon, Type I interferon; IFNA2; IFN-α 2a; Interferon alpha-A; LeIF A; Interferon alpha-2; Interferon alpha-2 Precursor; interferon, alpha 2

GeneID

3440

mRNA Refseq

NM_000605.2

Protein Refseq

NP_000596.2

MIM

147562

UniProt ID

P01563

Chromosome Location

9p22

Pathway

Antigen processing and presentation; Cytokine-cytokine receptor interaction ;Jak-STAT signaling pathway ;KEGG pathway: Natural killer cell mediated cytotoxicity ;Regulation of autophagy ;Toll-like receptor signaling pathway

Function

interferon-alpha/beta receptor binding