|
Cat. No.
|
CCL24-06H
|
|
Product Overview
|
Recombinant Human Eotaxin-2/CCL24 produced in E. coli is a single, non-glycosylated polypeptide chain containing 78 amino acids and having a molecular mass of 8.8 kDa.
|
|
Description
|
Eotaxin, also named MPIF-2 and Ckβ6, is a novel CC chemokine recently identified. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 selectively chemoattracts cells expressing CCR3 including eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells. Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells. The mature protein, which also includes a C-terminal truncation, contains 78 amino acid residues (92 a.a. residues for the murine homolog, without C-terminal truncation).
|
|
Purity
|
>97% by SDS-PAGE and HPLC analyses.
|
|
Formulation
|
Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Endotoxin
|
Less than 1EU/mg of rHuEotaxin-2/CCL24 as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20oC. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable for several weeks at 2-8oC, but should be kept at -20oC for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8oC. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20oC to -70oC. Avoid repeated freeze/thaw cycles.
|
|
Gene Information
|
|
Gene Name
|
CCL24
|
|
Synonyms
|
Ckb-6; MPIF2; MPIF-2; SCYA24; chemokine (C-C motif) ligand 24; eotaxin-2; MPIF2; CCL24_HUMAN; C-C motif chemokine 24 [Precursor]; Small-inducible cytokine A24; Myeloid progenitor inhibitory factor 2; CK-beta-6; Eosinophil chemotactic protein 2; Eotaxin-2; CCL24
|
|
GeneID
|
6369
|
|
mRNA Refseq
|
NM_002991
|
|
Protein Refseq
|
NP_002982
|
|
MIM
|
602495
|
|
UniProt ID
|
O00175
|
|
Chromosome Location
|
7q11.23
|
|
Pathway
|
Cytokine-cytokine receptor interaction.
|
|
Function
|
chemokine activity
|
| |
|
|
Download Datasheet:
|
|
Abbr.: 
