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Cat. No.
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CCL18-02H
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Product Overview
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Recombinant Human MIP-4/CCL18 produced in E. coli is a single, non-glycosylated polypeptide chain containing 69 amino acids and having a molecular mass of 7.8 kDa.
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Description
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CCL18, is a novel CC chemokine that is highly homologous to MIP-1α (61% amino acid sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10 and IL-13, and inhibited by IFN-γ. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells, but not monocytes or neutrophils.
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Purity
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>97% by SDS-PAGE and HPLC analyses.
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Formulation
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Lyophilized from a 0.2mm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 100mM NaCl.
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Specific Activity
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Fully biologically active when compared to standard. Determined by its ability to chemoattract human T lymphocytes using a concentration range of 1.0 -10.0 ng/ml.
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Endotoxin
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Less than 1EU/mg of rHuMIP-4/CCL18 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.
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Gene Information
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Gene Name
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CCL18
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Synonyms
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CKb7; PARC; AMAC1; DCCK1; MIP-4; AMAC-1; DC-CK1; SCYA18; CCL18_HUMAN; C-C motif chemokine 18 [Precursor]; Small-inducible cytokine A18;Macrophage inflammatory protein 4; ulmonary and activation-regulated chemokine;CC chemokine PARC;Alternative macrophage activation-associated CC chemokine 1; Dendritic cell chemokine 1; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated)
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GeneID
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6362
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mRNA Refseq
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NM_002988
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Protein Refseq
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NP_002979
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MIM
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603757
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UniProt ID
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P55774
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Chromosome Location
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17q11.2
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Pathway
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Cytokine-cytokine receptor interaction.
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Function
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chemokine activity.
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