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Cat. No.
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CAB045
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Antigen information
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Hepatitis B is one of a few known non-retroviralviruses which employ reverse transcriptionas a part of its replication process. (HIV, a completely unrelated virus, also uses reverse transcription, but it is a retrovirus.) HBV invades the cell by binding to surface receptor and become internalized. The viral core particles then migrate to the hepatocyte nucleus and the partially double-stranded, relaxed circular genomes (RC-DNA) are repaired to form a covalently closed circular DNA (cccDNA), which is the template for viral genomic and sub-genomic RNAs by cellular RNA polymerase II. Of these, the pregenomic RNA(pgRNA is selectively packaged into progeny capsids and is then reverse-transcribed into new RC-DNA. The core can either bud into the endoplasmic reticulum to be enveloped or exported from the cell or recycled back into the genome for conversion to cccDNA
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Specificity
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HBe-Ag
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Clone
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LT03
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Host Animal
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Mouse. Hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.
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Immunogen
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hepatitis virus granule
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Source
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Ascites
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Format
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Purified, Liquid
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Purification
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>90% pure (SDS-PAGE). Protein A chromatography
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Concentration
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4mg/ml (OD280nm, E0.1% = 1.4)
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Affinity Constant
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1X1011L/Mol
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Buffer
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PBS, pH 7.4
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Preservative
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0.1% NaN3
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Applications
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Detection for HBe antigen. Suitable for use in ELISA, Western-Blotting and tissue immunity, Suggested pair for ELISA immunoassay:
Capture Detection
L1H00401 L1H00402
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Storage
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Short term at 2-8°C,Long term at -20°C
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Download Datasheet:
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