Mouse Anti-Human Growth Arrest-specific 7 Monoclonal Antibody   [ Back  ]                         Price Inquiry

Cat. No.

CAB544

Antigen Information

Growth arrest-specific 7, also known as GAS7, is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development by promoting maturation and morphological differentiation of cerebellar neurons. Inhibition of GAS7 production in terminally differentiating cultures of embryonic murine cerebellum impedes neurite outgrowth. Conversely, the hyper-expression of GAS7 may play an important role in the initiation and development of human osteosarcoma.

Immunogen

Recombinant human GAS7 (1-416aa) purified from E. coli.

Isotype

Mouse IgG1 heavy chain and k light chain.

Clone

Anti-human GAS7 mAb, clone AT4H8, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GAS7 protein.

Concentration

1 mg/ml.

Form

Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Application

ELISA, WB.

Storage

Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Usage

The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1000. Recommended starting dilution is 1:500.

Western Blot Analysis

The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human GAS7 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Gene Information

Gene Name

GAS7 growth arrest-specific 7 [ Homo sapiens ]

Synonyms

GAS7; growth arrest-specific 7; MGC1348; KIAA0394; MLL/GAS7; Growth arrest-specific protein 7; GAS-7; MLL/GAS7 fusion protein

GeneID

8522

mRNA Refseq

NM_001130831

Protein Refseq

NP_001124303

MIM

603127

UniProt ID

O60861

Chromosome Location

17p13.1

Function

actin filament binding; protein binding; transcription factor activit