|
Cat. No. |
CAB376 |
|
Antigen
Information |
Epidermal
growth factor has a profound effect on the differentiation of
specific cells in vivo and is a potent mitogenic factor for a
variety of cultured cells of both ectodermal and mesodermal
origin. The EGF precursor is believed to exist as a
membrane-bound molecule which is proteolytically cleaved to
generate the 53-amino acid peptide hormone that stimulates cells
to divide. EGF stimulates the growth of various epidermal and
epithelial tissues in vivo and in vitro and of some fibroblasts
in cell culture. |
|
Immunogen |
r.HumanEGF. |
|
Ig Subclass |
Mouse IgG2b. |
|
Purification
Method |
Ion exchange. |
|
Protein
Concentration |
1mg/ml in PBS
(after reconstitution). |
|
Shipping
Conditions |
Antibody is
shipped lyophilized at ambient temperature. |
|
Reconstitution |
Reconstitute
with sterile H2O. Mix gently, wash the sides of the
vial and wait 30-60 seconds before use. |
|
Titer |
By direct
ELISA, 1:10,000 dilution will yield 0.3 O.D using alkaline
phosphatase conjugated rabbit anti-mouse Ig (Jackson
Laboratories). |
|
Applications |
Direct ELISA,
Western Blot, Immuneprecipitation.
|
|
Stability /
Shelf Life |
Two years lyophilized, one month in
solution at 4℃. |
|
Storage
Procedures |
In lyophilized form, for long
periods, store at 4℃
in a dry environment. After reconstitution, if not intended for
use within a month, aliquot and store at -20℃. |
|
Gene
Information
|
Gene Name |
EGF epidermal growth factor (beta-urogastrone) [ Homo sapiens ] |
|
Synonyms
|
EGF; epidermal
growth factor (beta-urogastrone) 21-Leu; URG; HOMG4;
Pro-epidermal growth factor; Epidermal growth factor;
Urogastrone |
|
GeneID |
1950 |
|
mRNA Refseq |
NM_001963 |
|
Protein Refseq |
NP_001954 |
|
MIM |
131530 |
|
UniProt ID |
P01133 |
|
Chromosome
Location |
4q25 |
|
Pathway |
Bladder cancer;
Cytokine-cytokine receptor interaction; Endocytosis; Endometrial
cancer; ErbB signaling pathway; Focal adhesion; Gap junction;
Glioma; MAPK signaling pathway; Melanoma; Non-small cell lung
cancer; Melanoma; Non-small cell lung cancer; Pancreatic cancer;
Pathways in cancer; Prostate cancer; Regulation of actin
cytoskeleton; Signaling by EGFR; Hemostasis |
|
Function |
calcium ion
binding; epidermal growth factor receptor binding; growth factor
activity; protein binding; transmembrane receptor protein
tyrosine kinase activator activity |
|
|
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Abbr.: 
