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Cat. No.
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CAB002
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Antigen information
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This gene ADH1A encodes class I alcohol dehydrogenase, alpha subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol,other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class I alcohol dehydrogenase, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. ADH1A gene is monomorphic and predominant in fetal and infant livers, whereas the genes encoding beta and gamma subunits are polymorphic and strongly expressed in adult livers.
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Immunogen
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Cytosolic proteins of human adult liver tissues (fraction 3)
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Isotope
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Mouse monoclonal IgG1κ
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Applications
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This antibody is effective in ELISA and Western blot assay.
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Specificity
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Recognizes native ADH1A (Homo spiens alcohol dehydrogenase alpha chain) at its molecular weight 40 kDa.
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Species Cross-reactivity
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Human. Others not determined.
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Formulation
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200μg of protein G purified IgG1κ in 172μLof 70% storage buffer (0.1M Tris-HCl, pH 7.4, 0.15M NaCl, 0.04% merthiolate) and 30% glycerol.
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Handling Recommendations
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Aliquot into microcentrifuge tubes and stored at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
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Recommended Working Dilution
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The optimal working dilution should be determined according to each specific assay condition.
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Gene Information
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Gene Name
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ADH1A
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Synonyms
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ADH1; ADH1A; alcohol dehydrogenase 1A (class I), alpha polypeptide; class I alcohol dehydrogenase, alpha subunit; ADH, alpha subunit; EC 1.1.1.1; ADH, alpha subunit; Alcohol dehydrogenase subunit alpha; alcohol dehydrogenase 1 (class I), alpha polypeptide.
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GeneID
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124
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mRNA Refseq
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NM_000667
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Protein Refseq
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NP_000658
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MIM
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103700
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UniProt ID
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P07327
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Chromosome Location
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4q21-q23
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Pathway
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1- and 2-Methylnaphthalene degradation; Bile acid biosynthesis; Fatty acid metabolism; Glycerolipid metabolism; Glycolysis / Gluconeogenesis; Metabolism of xenobiotics by cytochrome P450; Tyrosine metabolism; Biological oxidations.
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Function
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alcohol dehydrogenase activity; zinc-dependent; binding; metal ion binding; oxidoreductase activity; zinc ion binding.
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Alcohol dehydrogenase 1A (class I), alpha polypeptide
PDB rendering based on 1deh.
Available structures: 1deh, 1hdx, 1hdy, 1hdz, 1hso, 1hsz, 1ht0, 1htb, 1u3t, 1u3u, 1u3v, 1u3w, 3hud
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Download Datasheet:
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