|
From Microorganism
EC 1.5.3.1
|
Cat. No.
|
CED023
|
|
Description
|
Sarcosine oxidase (SAO) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2, 5,10-CH2-tetrahydrofolate in a reaction requiring H4-tetrahydrofolate and oxygen.
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2
|
|
CAS No.
|
9029-22-5
|
|
Synonym
|
L-Pipecolic Acid Oxidase; Sarcosine-Oxygen Oxidoreductase (Demethylating); Oxidoreductases, N-Demethylating
|
|
Physical form
|
Yellowish powder, lyophilized
|
|
Specific Activity
|
≥8U/mg protein
|
|
Unit definition
|
One unit causes the formation of one micromole of hydrogen peroxide per minute at pH 8.0 at 37℃.
|
|
Molecular Weight
|
65 kDa (Gel filtration)
|
|
Isoelectric point
|
5.0
|
|
Michaelis constant
|
2.7×10-3M(Sarcosine)
|
|
Optimum pH
|
8.0
|
|
Optimum temperature
|
40℃
|
|
pH Stability
|
7.0~9.0 (25°C,20hr)
|
|
Storage Temperature
|
-20°C
|
|
Thermal Stability
|
<40℃ (pH7.0, 30min)
|
|
Inhibitors
|
Ag+, Hg2+, Cu2+ are strongly inhibiting. The even stronger inhibiting effect of thiol group suggests that SDS might play a role in the active site of the enzyme.
|
|
Preparation Instructions
|
The enzyme is reconstituted in 20mM Tris-HCl buffer, pH 8.0 for activity assay.
|
|
Gene Information
|
Gene Name
|
SAO (Bos taurus)
|
|
Gene Alias
|
BSAO
|
|
GeneID
|
789307
|
|
mRNA Refseq
|
NM_001130764
|
|
Protein Refseq
|
NP_001124236
|
|
UniProt ID
|
Q29437
|
|
Chromosome Location
|
19
|
|
Function
|
Amine oxidase activity; oxidoreductase activity; calcium ion binding; copper ion binding;quinone binding
|
|
|
Download Datasheet:
|
|
|
