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From Microorganism
EC 3.5.2.10
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Cat. No.
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CED008
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Description
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Creatinine Amidohydrolase catalyzes the hydrolytic reaction converting creatinine to creatine. The enzyme is purified from a microorganism. The molecular size of the enzyme is approximately 175,000. The enzyme is useful for the enzy-matic assay of creatinine when coupled with other related enzymes.
Creatinine + H2O → Creatine.
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CAS No.
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9025-13-2
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Synonym
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creatininase; creatinine hydrolase; creatinine amidohydrolase.
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Physical Form
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White powder, lyophilized
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Specific Activity
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≥ 250U/mg protein
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Unit Definition
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One unit will convert one micromole of creatinine to creatine per min at pH 7.5 at 37℃.
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Molecular Weight
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160 kDa (Gel filtration)
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Isoelectric Point
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pH4.7
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Michaelis Constant
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5.2×10-2M(Creatinine)
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Optimum pH
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6.0~ 8.0
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Optimum Temperature
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80℃
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pH Stability
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7.0~9.0 (25℃, 20hr)
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Storage Temperature
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-20℃
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Thermal Stability
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< 60℃ (pH7.5, 60min)
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Inhibitors
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Ag+, Hg2+. EDTA
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Preparation Instructions
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The enzyme is reconstituted in 50mM potassium phosphate buffer, pH 7.5 for activity assay.
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Gene Information
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Gene Name
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GSU1722 creatinine amidohydrolase (Geobacter sulfurreducens PCA)
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GeneID
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2685478
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mRNA Refseq
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NP_952772
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Function
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hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides.
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Download Datasheet:
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