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Cat. No.
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CED005
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Product Overview
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Recombinant Cholesterol Oxidase produced in E.coli has a molecular mass of about 34,000 Da.
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Description
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Recombinant Cholesterol Oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. This enzyme participates in bile acid biosynthesis.
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CAS No.
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9026-00-0
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Synonym
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Cholesterol- O2 oxidoreductase; 3beta-Hydroxy steroid oxidoreductase; 3β-hydroxysteroid:oxygen oxidoreductase; cholesterol:oxygen oxidoreductase; cholesterol oxidase.
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Reaction
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cholesterol + O2 = cholest-4-en-3-one + H2O2
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Activity
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Grade Ⅲ 15 U/mg-solid or more(contg. approx. 40% of stabilizers)
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Appearance
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Yellowish amorphous powder, lyophilized
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Contaminants
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Catalase ≤1.0%; Cholesterol esterase ≤1.0×10-2%
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Additives
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Bovine serum albumin, sugars
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Molecular weight
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about 34,000 (gel filtration)
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Isoelectic point
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5.1±0.1 and 5.4±0.1
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Michealis constants
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2.1×10-2 M (Creatine)
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Structure
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dimer
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Inhibitors
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onic detergents, Hg2＋, Ag
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Optimum pH
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6.5~7.0
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Optimum temperature
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45~50℃
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pH stability
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pH 5.0~10.0 (25℃, 20hr)
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Thermal stability
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below 45℃ (pH 7.0, 15min)
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Stability
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Stable at -20℃ for at least 6 months
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Download Datasheet:
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