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Cat. No.
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CED001
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Description
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Adenosine deaminase (ADA) is a purine catabolic enzyme ubiquitous in mammalian tissue. In vitro it catalyzes deamination of both adenosine and 2'-deoxyadenosine to inosine and 2'-deoxyinosine respectively.
Adenosine + H2O → Inosine + NH3.
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CAS No.
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9026-93-1
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Synonym
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Adenosine aminohydrolase; deoxyadenosine deaminase.
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Physical form
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Suspension in 3.2 M (NH4)2SO4, 0.01 M potassium phosphate, pH 6.0;
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Specific Activity
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150~200U/mg protein
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Unit definition
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One unit will deaminate one micromole of adenosine to inosine per minute at pH 7.4 at 25℃.
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Molecular Weight
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32.5~33 kDa (SDS-PAGE)
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Isoelectric point
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pH 4.85
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pH Stability
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4.0~10.0
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Composition
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The enzyme is a glycoprotein containing galactosamine and glucosamine. Its composition shows a predominance of acidic amino acids.
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Optimum pH
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7.4
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Optimum temperature
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75℃
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Storage Temperature
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2-8℃(Stable≥6 months)
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Thermal Stability
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<60℃ (pH7.4, 30min)
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Inhibitors
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Ag+, Hg2+, Cu2+, sulfhydryl reagents (pCMB and methyl mercuric nitrate) are strongly inhibiting. The even stronger inhibiting effect of TPCK and DFP suggests that histidine or serine or both might play a role in the active site of the enzyme.
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Preparation Instructions
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The enzyme preparation may be diluted in water or buffer to a protein concentration of 1mg/ml, producing a clear, colorless solution.
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Gene Information
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Gene Name
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ADA (Human)
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GeneID
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100
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mRNA Refseq
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NM_000022
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Protein Refseq
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NP_000013
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UniProt ID
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P00813
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Chromosome Location
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20q12-q13.11
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Pathway
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Purine metabolism; Nucleotide metabolism
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Function
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adenosine deaminase activity; hydrolase activity; protein binding; zinc ion binding
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Ribbon diagram of bovine adenosine deaminase.
Zinc ion visible at center. From PDB 1VFL
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Download Datasheet:
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