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Cat. No.
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CPE09
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Description
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Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-b-D-glucose and D-glucose residues in hyaluronate.
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CAS NO.
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37326-33-3
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Molecular weight
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Borders and Raftery reported a molecular weight of 61,000. Khorlin et al. report four subunits of 14,000 each and a total molecular weight of 55,000.
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Synonyms
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Hyaluronoglucosaminidase; hyaluronidase; hyaluronoglucosidase; chondroitinase; chondroitinase I; hyaluronate 4-glycanohydrolase
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Reaction
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Random hydrolysis of 14-linkages between N-acetyl-β-D-glucosamine and D-glucuronate residues in hyaluronate
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Unit definition
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1 unit equals a U.S.P./National Formulary Unit and is referenced to a standard NF Hyaluronidase.
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Extinction coefficient
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approximately 8.
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Specificity
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Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues. Monosaccharides are not liberated.
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Composition
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The enzyme is a glycoprotein containing 5% mannose and 2.17% glucosamine. The amino acid composition has been determined.
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Optimum pH
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4.5 - 6
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Inhibitors
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Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+
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Stabilizers
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sodium chloride acts as a stabilizer.
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Stability/Storage
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hyaluronidase is stable for 1 - 2 years and the purified preparation for 6 - 12 months when stored at 2 -8°C.
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Product Specification
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Appearance
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A white or yellowis-white powder
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Identification (EP)
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Complies
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Appearance of solution(EP)
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Clear
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Loss on drying(EP)
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≤ 5.0%
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Bacterial endotoxins(EP)
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Less than 0.2 IU per IU of hyaluronidase
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Limit of tyrosine( USP)
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≤ 0.25 μg per USP Hyaluronidase U.
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Activity (EP)
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≥ 1000 IU/mg powder
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pH(EP)
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4.5 – 7.5
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Conclusion: Product complies with USP and EP.
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Hyaluronidase
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Download Datasheet:
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Abbr.: 
